Animal cells and systems

  • 제2권1호
  • /
  • Pages.123-131
  • /
  • 1998
  • /
  • 1976-8354(pISSN)
  • /
  • 2151-2485(eISSN)
한국통합생물학회 (The Korean Society for Integrative Biology)
권호 표지

Isolation and Characterization of Lethal Mutation near the unc-29 (LG I) Region of Caenorhabditis elegans

  • Lee, Jin-Sook (Department of Life Science, Kwangju Institute of Science and Technology) ;
  • An, Joo-Hong (Department of Life Science, Kwangju Institute of Science and Technology)
  • 발행 : 1998.03.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The unc-29 region on the chromosome I of Caenorhabditis elegans has been mutagenized in order to obtain lethal mutations. In this screen, the uncoordinated phenotype of unc-29 (e193) mutant was used to identify any lethal mutations closely linked to the unc-29 gene, which encodes a subunit of nicotinic acetylcholine receptors. We have isolated six independent mutations (jh1 to jh6) out of approximately 5,200 ethyl methanesulfonate(EMS) treated haploids. Four of the six mutations demonstrated embryonic lethal phenotypes, while the other two showed embryonic and larval lethal phenotypes. Terminal phenotypes observed in two mutations (jh1 and jh2) indicated developmental defects specific to posterior part of embryos which appeared similar to the phenotypes observed in nob (no back end) mutants. Another mutation (jh4) resulted in an interesting phenotype of body-wall muscle degeneration at larval stage. These mutations were mapped by using three-factor crosses and deficiency mutants in this region. Here we report genetic analysis and characterization of these lethal mutations.

키워드

참고문헌

  1. Genetics v.137 A screen for genetic loci required for body wall muscle development during embryogenesis in Caenorhabditis elegans Ahnn JH;Fire A
  2. Cell. v.54 Duchenne muscular dystrophy: deficiency of dystrophin at the muscle cell surface Bonila E;Samitt CE;Rowland LP
  3. Genetics v.77 The genetics of Caenorhabditis elegans Brenner S
  4. Nature v.345 The identification and suppression of inherited neurodegeneration in a Caenorhabditis elegans Brenner S
  5. Nature v.349 The mec-4 gene is a member of a family of Caenorhabditis elegans genes that can murate to induce neuronal degeneration Driscoll M;Chalfile M
  6. Cell. v.66 Membrane organization of the dystrophin-glycoprotein complex Ervasti JM;Campbell KP
  7. Genetics v.110 Identification and characterication of 22 genes that affect the vulval cell lineages of the nematode Caenorhabditis elegans Ferguson EL;Horbitz HR
  8. EMBO J. v.5 Integrative transformation of Carnorhabditis elegans Fire A
  9. Comp. Mol. Neurobiol. v.63 Acethlcholine receptor molecules of the nematode Canorhabditis elegans Fleming JT;Tornoe C;Riina HA;Coadwell J;Lewis JA;Sattlle DB
  10. J. Neurosci. v.17 Caenorhabditis elegans levamisole resistance genes lev-1, unc-29, and unc-39 encode functional nicotinic acetyl-cholin ereceprot subunits Flerming JT;Squire MD;Barnes TM;Tornoe C;Matsuda K;Ahnn JH;Sulston JE;Barnard EA;Sattlle DB;Lewis JA
  11. C. elegans Ⅱ Cell death Hengatner MO;Riddle DL(eds.);Blumental T(eds.);Meyer BJ(eds.);Priess RJ(eds.)
  12. Cell. v.51 Dystrophin: the protein product of the Duchenne muscular dystrophy locus Hoffman EP;Brown RH;Kunkel LM
  13. Korean J. Biol. Sci. v.1 A screen for genetic loci on the X chromosome required for body-wall muscle development during embryogenesis in Caenorhabditis elegans Lee DG; Shin JY;Ahnn JH
  14. Genetics v.95 Teh genetics of levamisole resistance in the nematode Caenorhabditis elegans Lewis JA;Wu CH;Berg H;Levine JH
  15. Neeuro-science v.5 Levamisoleresistant mutants of the nematode Caenorhabditis elgans appear to lack pharmacological acetylcholine receptors Lewis JA;Wu CH;Levine JH;Berg H
  16. Methods in Cell Biology. Caenorhabditis elegans: Modern Biological Analysis of anorganism v.48 DNA transformation Mello C;Fire A;Epstein HF(eds.);Shakes DC(eds.)
  17. J. Phys. Radium. v.16 Microinterferomettre differentiel a ondes polarisees Nomarski G
  18. C. elegans Ⅱ Developmental genetics of the germline Scheedl T;Riddle DL(eds.);Blumental T(eds.);Meyer BJ(eds.);Priess RJ(eds.)
  19. Dev. Biol. v.100 The embryonic cell lineage of the nematode Caenorhabditis elegans Sulston JE;Schirerenberg E;White JG;Thomson JN
  20. The Nematode C. elegans Method Sulston JE;Hodgkin J;Wood WB(ed.)
  21. Neuron. v.14 A mutated acetylcholine receptor subunit cause neuronal degeneration in C. elegans Treinin M;Chalfie M
  22. The Nematode C. elegans Introduction to C. elegans biology Wood WB;Wood WB(ed.)
  23. Dev. Biol. v.74 Parental effects and phenotypic characterization of mutations that effect early development in Caenorhavditis elegans Wood WB;Hecht R;Carr S;Vanderslice R;Wolf N;Hirsh D