Photosynthetic Responses to Dehydration in Green Pepper(Capsicum annuum L.)Leaves

  • Published : 1998.12.01

Abstract

Photosynthetic responses to dehydration were examined by the simulataneous measurement of O2 evolution and chlorophyll (Chl) fluorescence in green pepper leaves. Dehydration was induced by immersing the plant roots directly in the Hoagland solution containing varying concentration (2-30%) of polyethylene glycol(PEG-6000) . Water potential of the leaf was decreased time-and concentation -dependently by PEG-treatment. The decrease in water potential of leaf was correlated with the decrease in both the maximal photosynthesis (Pmax) and quantum yield of O2 evolution, but Pmax dropped more rapidly than quantum yield at all water deficit conditions tested. However, Chl fluorescence parameters were not affected much. Dehydration did not change the initial fluorescence (Fo) and maximum photochemical efficiency(Fv/Fm) of photosystem(PS) II. Both the photochemical quenching (qP) and non-photochemical quenching(NPQ) were not changed by dehydration under low PFR(50 $\mu$mols m-2s-1 ). In contrast, under high PFR(270$\mu$mols m-2s-1)qP was slightly decreased while NPQ was greatly increased. The fast induction kinetics of Chl fluroecence showed no change in Chl fluorescence pattern by dehydration at high PFR (640 $\mu$mols m-2s-1 ), but exhibited a significant drop in peak level(Fp)at low PRFR (70$\mu$mols m-2s-1 ). PS I oxidation and reduction kinetics revealed normal reduction but delayed oxidation to P-700+, suggesting no lesionin electron flow from PSII to PSI , but impaired electron transport to NADP+,These results suggest that water stress caused by PEG-treatment results in the reduction of photosynthesis, promarily due to the reducted electron trasport from PSI to NADP+ or hampered subsequent steps involving Calvin Cycle.

Keywords

References

  1. Stress and stress coping in cultivated plants McKersie, B. D.;Y. Y. Leshem
  2. Water Deficits and Plant Growth v.Ⅳ Water deficits and photosynthesis;Soil Water Measurement, Plant Responses, and Breeding for Drought Resistance Boyer, J. S.;T. T. Kozlowski(Ed.)
  3. Planta v.161 Inhibition of photosynthetic reactions under water stress : interaction with light level Bjorkman, O.;S. B. Powles
  4. Planta v.166 Effects of drought on photosynthesis, chlorophyll fluorescence and photoinhibition susceptibility in intact willow leaves Ogren, E. ;G. Oquist
  5. Plant Cell Environ v.11 Stomatal and non-stomatal limitations of carbon metabolism: an evaluation of the path-dependent method Assman, S. M.
  6. Ann. Rev. Plant Physiol. v.33 Stomatal conductance and photosynthesis Farquhar, G. D.;T. D. Sharkey
  7. Physiol. Plant. v.71 Effects of water deficit on photosynthetic capacity Kaiser, W. M.
  8. Phaseolus vulgaris L. and Elatostema repens. Planta v.177 Effect of dehydration and high light on photosynthesis of two C3 plants Cornic, G.;J. L. Le gouallec;J. M. Briantais;M. Hodges
  9. J. Exp. Bot. v.42 Effects of water deficits on carbon assimilation Chaves, M. M.
  10. Plant Adaptation to Environmental Stress Plants under salt and water stress Shalhevet, J.
  11. Plant Physiol. v.45 Inhibition of oxygen evolution in chloroplasts isolated from leaves with low water potentials Boyer, J. S.;B. L. Bowen
  12. Plant Physiol v.57 Chloroplast response to low water potentials.Ⅳ. Quantum yield is reduced Mohanty, P.;J. S. Boyer
  13. Nature v.213 Effect of water stress on the photochemical activity of chloroplasts Nir, I.;A. Poljakoff-Mayber
  14. Plant Physiol. v.53 Chloroplast respones to low water potentials. Ⅲ. Differing inhibition of electoon transport and photophosphorylation Keck, R. W.;J. S. Boyer
  15. Physiol. Plant. v.93 Effects of water stress on photochemical function and protein metabolism of photosystem Ⅱ in wheat leaves Jun, X. H.;J. Wang;H. G. Liang
  16. Can. J. Bot. v.56 Chlorophyll fluorescence induction: an indicator of photosynthetic activity in marine algae undergoing desiccation Wiltens, J.;U. Schreiber;W. Vidaber
  17. Plant Physiol. v.82 Photosynthetic responses to water stress in leaves, expressed by photo-acoustics and related methods. Ⅱ. The Effect of rapid drought on the electron transport and the relative activities of the two photosystem Havaux, M.;O. Canani;S. Malkin
  18. Planta v.160 Effects of partial defoliation, changes of irradiance during growth, shortterm water stress and growth at enhanced p(CO₂) on the photosynthetic capacity of laves of Phaseolus vulgaris L. von Caemmerer, S.;G. D. Farquhar
  19. Planta v.153 Photosynthesis under osmotic stress. Effect of high solute concentrations on the permeability properties of the chloroplast envelope and on the activity of stromal enzymes Kaiser, W. M.;U. Hever
  20. Plant Physiol. v.70 Effect of osmotic stress in photosynthesis studied with isolated chloroplast. Site-specific inhibition of the photosynthetic carbon reduction cycle Berkowitz, G. A.;M. Gibbs
  21. Plant Physiol. v.72 Reduced osmotic potential inhibition of photosynthesis. Site-specific effects of osmotically induced stromal acidification Berkowitz, G. A.;M. Gibbs
  22. Plant Sci. Lett. v.20 Chlorophyll a fluorescence transient as an indicator of water potential of leaves Govindjee, W. S.;Downton, D. C. Fox;P. A. Armond
  23. Plant Physiol. v.82 Photosynthetic responses to water stress in leaves, expressed by photoacoustics and related method. Ⅰ. Probing the photacoustic method as an indicator for water stress in vivo. Havaux, M.;O. Canani;S. Malkin
  24. Plant Physiol. v.45 Carbowax 6000 compared with mannitol as a suppressant of cucumber hypocotyl elongation Michel, B. E.
  25. Plant Physiol. v.48 Further comparisons between Carbowax 6000 and mannitol as suppressants of cucumber hypocotyl elongation Michel, B. E.
  26. Proc. Nat. Acad. Sci. U.S.A. v.54 Isopiestic technique for measuring leaf water potentials with a thermocouple psychometer Boyer, J. S.;E. B. Knipling
  27. Ecophysiology of Photosynthesis Chlorophyll fluorescence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis Schreiber, U.;W. Bilger;C. Neubauer;E.-D. Schulze(ed.);M. M. Caldwell(ed.)
  28. Biochim. Biophys. Acta v.975 Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy Porra, R. J.;W. A. Thompson;P. E. Kriedemann
  29. The use of the oxygen electrode and fluorescence probes in simple measurements of photosynthesis Walker, D.
  30. Light Emission by Plants and Bacteria Practical applications of fluometric methods to algae and higher plant research Renger, G.;U. Schreiber;Govindjee, J. Amesz(ed.);D. C. Fork(ed.)
  31. Aust. J. Plant Physiol. v.15 Effects of photoinhibition induced by water stress on growth and yield of grain sorghum Ludlow, M. M.;S. B. Powles
  32. J. Exp. Bot. v.47 Inhibition of photosynthesis in olive tree (Olea europaea L.) during water stress and rewatering Angelopoulos, K.;B. Dichio;C. Xiloyannis
  33. Plant Cell Environ v.9 The use of light-induced absorbance charges at 820 nm to monitor the oxidation state of P-700 in the leaves Harbinson, J.;F. I. Woodward
  34. Plant Cell Environ v.12 The kinetics of P-700+ reduction in leaves: a novel in situ probe of thylakoid functioning Harbinson, J.;C. L. Hedley