대한한방내과학회지 (The Journal of Internal Korean Medicine)

대한한방내과학회 (The Society of Internal Korean Medicine)
권호 표지

전갈(全蝎)이 뇌조직(腦組織)의 $Na^+-K^+-ATPase$ 활성(活性)에 미치는 영향(影響)

Effect of Buthus on $Na^+-K^+-ATPase$ activity in cerebral synaptosomes

  • 윤종영 (동국대학교 한의과대학 내과학교실) ;
  • 신현철 (동국대학교 한의과대학 내과학교실) ;
  • 윤철호 (동국대학교 한의과대학 내과학교실) ;
  • 서운교 (동국대학교 한의과대학 내과학교실) ;
  • 김종호 (동국대학교 한의과대학 내과학교실) ;
  • 정지천 (동국대학교 한의과대학 내과학교실)
  • Yoon, Jong-Yeong (Dept. of Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Shin, Hyeon-Chul (Dept. of Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Yoon, Chul-Ho (Dept. of Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Seo, Un-Kyo (Dept. of Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Kim, Jong-Dae (Dept. of Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Jeong, Ji-Cheon (Dept. of Internal Medicine, College of Oriental Medicine, Dongguk University)
  • 발행 : 1998.08.30
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초록

This study was undertaken to determine whether Buthus exract(BTE) affects Na^+-K^+-ATPase$ activity of nervous tissues. The enzym activity was measured in synaptosomal fraction prepared from rabbit brain cortex. Na^+-K^+-ATPase$ activity was inhibited by BTE over concentration range of 0.05-0.5% in a dose-dependent manner. The enzyme activity was increased by an increase in $Na^+$ concentration from 5 to 100mM, $K^+$ concentration from 0.5 to 10mM, and $Mg^{2+}$ concentration from 0.2 to 5mM. These changes in ion concentrations did not produce any effect on the inhibitory effect of BTE on $Na^+-K^+-ATPase$ activity. An increase in ATP concentration from 0.1 to 3mM caused an increase in the enzyme activity. The inhibition of the enzyme activity by BTE were not different between two ATP concentrations. A sulfhydryl group protector DTT prevented PCMB-induced inhibition of $Na^+-K^+-ATPase$ activity, but the BTE-induced inhibition was not altered by DTT. The inhibition of enzyme activity by combination of ouabain and BTE was not different from that by Buthus alone. These results suggest that Buthus exerts inhibitory effect on $Na^+-K^+-ATPase$ activity in cerebral synaptosomes, and the action mechansim is similar to that of ouabain.

키워드

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