The effect of Astragali Radix and Armeniacae Semen on the apoptosis of immunocytes and nitric oxide production from peritoneal macrophages

황기와 행인(杏仁)이 면역세포(免疫細胞)의 Apoptosis 및 Nitric Oxide에 미치는 효과(效果)

  • Published : 1998.11.30

Abstract

The purpose of this research was to investigate effects of Astragali Radix(AR) and Armeniacae Semen(AS) on T-lymphocytes and peritoneal macrophages in mice. The proliferation of thymocytes and splenocytes were teated using macroplate-reader. The apoptosis and sub-population of T-lymphocytes were tested using a flow cytometer. Nitric oxide production was tested using a Griess reagents. The result were obtained as follow; 1. AR increased the proliferation of thymocytes and splenocytes. 2. AS decreased the proliferation of thymocytes and splenocytes. 3. AR and AS decreased No production fron peritoneal macrophages 4. AR and AS were accelerate T-lymphocytes apoptosis. 5. AR and AS increased $T_C$ cells population, but decreased $T_H$ cells population of T-lymphocyte.

Keywords

References

  1. 黃帝內經素問靈樞譯解 楊維傑
  2. 臨床本草學 辛民敎
  3. 中藥藥理與應用 王浴生
  4. 申氏本草學 申佶求
  5. 일반병리학 김상호(외4인)
  6. 免疫學 하대유(외25人)
  7. 托裏消毒飮이 마우스의 綿羊赤血球에 대한 免疫反應에 미치는 影響 安大宗
  8. 養心湯이 스트레스와 免疫機能에 미치는 影響 尹祥熙
  9. 補中益氣湯의 投與가 紫外線 照射로 低下된 마우스의 免疫機能의 恢復에 미치는 影響 閔勇泰
  10. 生地黃의 投與가 마우스의 先天免疫 및 適應免疫 反應에 미치는 影響 椎榮坦
  11. 鍼灸刺戟이 생쥐의 免疫系에 미치는 影響 嚴泰植
  12. 大韓本草學會誌 v.12 no.1 數種 補益劑가 免疫細胞의 調節 및 Apoptosis에 미치는 影響 韓宗鉉(外5人)
  13. 大韓韓方內科學會誌 v.18 no.2 竹瀝이 T-lymphocyte rte 및 腹腔 Macrophage에 미치는 影響 鄭鉉雨(外5人)
  14. J. Immunol. methods. Mosmann, T.
  15. Kotnic, V.;Fleischmann, W.R.Jr. Kotnic, V.;Fleischmann, W.R.Jr.
  16. Proc. Natl. Acad. Sci. USA. Wysocki, L. J.;Sato, V. L.
  17. J. Immunol. Mizel, S. B.;Openheim, J. J.;Rosensteich, D. L.
  18. Infec. Imumnity v.59 no.9 Killing of Plasmodium faciparum in vitro by nitric oxide derivatives. Rockett K,A.;Awbum, M. M.;Cowden, W. B.;Clark, I. A.
  19. J. Immunol. Methods Rapid and simple method for mesuring thymocyte apoptosis by propidium iodide staining and flow cytometry. I. Nicoletti;G. Migliorati;M. C. Pagliacci;F. Grignani;C. A. Riccardi
  20. Science Macrophage cytotoxicity role for L-arginine deaminase and imino nitrogen oxidation to nitrite. Hibbs, J. B.;Taintor, R. R.;Vavrin, Z.
  21. Jpn. J. Cancer Res. Leukemic cell lysis by activated human macrophages. Nakabo, Y.;Harakawa, N.;Yamamoto, K.;Okuma, M.;Uno, K.;Sasada, M.
  22. J. Immunol. v.14 Kinetic study of antibody producing cells in the spleen of mice immuized intravenously with sheep erythrocytes Biozzi G;Stiefel C;Moutorl Bouthillier Y;Deceusefound GA.
  23. Medicine Immunology v.12 Anti-tumor effect of macrophage Ymazaki M.
  24. Essential Immunology Roitt l.
  25. Immunol. Today. Cellular signaling in programmed cell death (apoptosis). McConkey, D.J.(et al)
  26. Nature Antibodies to CD/T-cell receptor complex induce death by apoptosis in immature T cells in thymic cultures. Smith, C. A.;Wiliiams, G. T.;Kingston, E.; Jenkinson, J.;Owen, J. J. T.
  27. J. Exp. Med. Reduction in mitochondrial potential constitutes an early irreversible step of programmed lymphocyte death in VIVO. Zamzami, N.;Marchetti, P.;Castedo, M.;Zanin, C.;Vayssiere, J. L.;Petit, P. X.;Kroemer, G.
  28. J. Exp. Med. Prevention of agerelated T cell apoptosis defect in CD2- fas- transgenic mice. Zhou, T.;Edwards, C. K.;Mounts, J. D.
  29. J. Immunol. v.144 Cytolytic mechanisms of activated macrophages ; Tumor necrosis factor and L-arginine- depenent mechanisms act synergistically as the major cytolytic mechanism of activated macrophages Higuchi MM;Hlgashi N;Taki H;Osawa T.