Aeromonas hydrophila KH-54가 분비하는 유기폐수처리용 생물응집제 생산조건의 최적화

Optimization of Bioflocculant Production Conditions for Organic Wastewater Treatment with Aeromonas hydrophila KH-54

미생물이 생산하는 유기폐수처리용 생물응집제를 개발하기 위하여 보관균주 및 자연계로부터 100 units/ml 이상의 응집활성을 나타내는 응집제 생산균주를 1차로 분리하고 돈분폐수에서 가장 높은 제거율을 보인 KH-54를 최종 선별하였다. 선별된 균주는 Aeromonas hydrophila로 동정되었으며 응집제 생산을 위한 최적 배양조건은 2.0% mannitol, 0.05% ammonium chloride, 0.02% $KH_2PO_4$, 0.01% $MgSO_4{\cdot}7H_2O$, 0.05% yeast extract 조성의 배지와 초기 pH 7.0, 배양온도 $25^{\circ}C$ 및 교반속도 150 rpm 의 환경인자들이었다. 상기의 조건으로 4일 배양하였을 때 770 units/ml의 응집활성과 81%의 높은 nephelometer turbidity unit(NTU) 제거율을 나타내었으며 이 응집물질은 돈분폐수 이외 두부공업폐수 92%, 제당폐수 78%, 주정공업폐수 68% 및 항생물질 발효공업폐수 36%의 NTU 제거율을 각각 나타내었다.

For the need of bio-degradable flocculant in stage of wastewater treatment, some cultural conditions of bioflocculant production were optimized with Aeromonas hydrophila KH-54. About 260 strains of type culture and bacteria isolated from marsh, pond, activated sludge, etc were examined for their ability to flocculate kaolin particles and swine wastewater. Among them, KH-54 showed the highest flocculating activity and was identified as Aeromonas hydrophila according to the cultural, morphological and physiological properties. The maximum production of the flocculant secreted by Aeromonas hydrophila KH-54 was observed in culture medium containing 2.0% mannitol, 0.05% ammonium chloride, 0.02% potassium phosphate dibasic, 0.01% $MgSO_4{\cdot}7H_2O$ and 0.05% yeast extract at initial pH 7.0 when cultured on rotary shaker controlled at $25^{\circ}C$ and 150 rpm. Under the optimized condition, the flocculating ability reached to 770 units/ml of kaolin flocculating activity and 81% of NTU removal efficiency against swine wastewater after 4 days cultivation. The bioflocculant was also effective on various organic wastewaters other than swine wastewater, showing NTU removal rate ranging from 92% to 34%.