Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Cytotoxicity of steroid-saponins from the tuber of Liriope platyphylla W. T.

맥문동(Liriope platyphylla W. T.) 스테로이드 사포닌의 항암활성

  • Cho, Sung-Ji (Taegu Hyosung Catholic University, College of Pharmacy) ;
  • Bang, Myun-Ho (Kyung-Hee University, Department of Life Science & Resources, Institute of Life Science & Resources) ;
  • Lee, In-Ja (Taegu Hyosung Catholic University, College of Pharmacy) ;
  • Park, Chang-Gi (Youngnam Agricultural Institute) ;
  • Kim, Moo-Sung (Kyung-Hee University, Department of Life Science & Resources, Institute of Life Science & Resources) ;
  • Kim, Keum-Sook (Youngnam Agricultural Institute) ;
  • Sung, Jae-Duk (Youngnam Agricultural Institute) ;
  • Baek, Nam-In (Kyung-Hee University, Department of Life Science & Resources, Institute of Life Science & Resources)
  • 조성지 (대구효성카톨릭대학교 약학대학) ;
  • 방면호 (경희대학교 생명자원과학부 및 생명자원과학연구원) ;
  • 이인자 (대구효성카톨릭대학교 약학대학) ;
  • 박창기 (영남농업시험장) ;
  • 김무성 (경희대학교 생명자원과학부 및 생명자원과학연구원) ;
  • 김금숙 (영남농업시험장) ;
  • 성재덕 (영남농업시험장) ;
  • 백남인 (경희대학교 생명자원과학부 및 생명자원과학연구원)
  • Published : 1998.08.31
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Abstract

The tuber of Liriope platyphylla was extracted with 80% aqueous MeOH and solvent-fractionated with EtOAc, n-BuOH and $H_2O$. Evaluation of growth inhibitory activity of each fractions on various carcinoma cells, A549, SK-OV-3, SK-Mel-2, XF-498 and HCT-15, indicated the n-BuOH fraction to be the highest in the activity. From the fraction, two saponin compounds were isolated through Amberlite XAD-II and silica gel column chromatographies, repeatedly, and their chemical structures were elucidated as spicatoside A and B by interpretation of spectral data, NMR and IR, and adaptation of acid hydrolysis. Spicatoside A showed growth inhibitory activity on carcinoma cells, and the $IC_{50}$ values against A549, SK-OV-3, SK-Mel-2, XF-498 and HCT-15 cells were determined to be 17.3, 21.7, 14.9, 18.8 and $15.6\;{\mu}g/ml$, respectively.

맥문동 괴근을 80% MeOH 수용액으로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 $H_2O$로 용매 분획하였다. 암세포 A549, SK-OV-3, SK-Mel-2, XF-498 및 HCT-15에 대한 각 분획물의 생장억제활성을 SRB 법으로 측정한 결과 n-BuOH 분획에서 암세포 생장억제활성이 높게 나타났다. n-BuOH 분획으로부터 Amberlite XAD-II 및 silica gel column chromatography를 반복하여 2종의 사포닌을 분리하였고, NMR, IR 데이터의 해석과 가수분해반응을 이용하여 spicatoside A와 B로 동정하였다. Spicatoside A가 암세포에 대한 생장억제활성을 보여 A549, SK-OV-3, SK-Mel-2, XF-498 및 HCT-15에 대한 $IC_{50}$ 값이 각각 17.3, 21.7, 14.9, 18.8 및 $15.6\;{\mu}g/ml$를 나타내었다.

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