Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Cytotoxic Activity of Leguminous Seed Extracts against Human Tumor Cell Lines

  • Lee, Hoi-Seon (Division of Applied Biology and Chemistry, and the Research Center for New Bio-Materials in Agriculture, College of Agriculture and Life Sciences, Seoul National University) ;
  • Lee, Jeong-Ock (Korea Research Institute of Chemical Technology) ;
  • Lee, Hee-Kwon (National Institute of Sericulture and Entomology, Rural Development Administration) ;
  • Oh, Jong-Hwan (Forestry Research Institute) ;
  • Ahn, Young-Joon (Division of Applied Biology and Chemistry, and the Research Center for New Bio-Materials in Agriculture, College of Agriculture and Life Sciences, Seoul National University)
  • Published : 1998.06.30
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Abstract

The cytotoxic activity of methanol extracts of 25 leguminous seeds in vitro was evaluated by sulforhodamine B assay, using the five human solid A549 lung, SK-OV-2 ovarian, SK-MEL-2 melanoma, XF-498 CNS and HCT-15 colon tumor cell lines. The responses varied with both cell line arid leguminous seed used. Extracts of Canavalia lineata and Glycine soja revealed potent cytotoxic activity against A549 arid SK-MEL-2 cell lines. Moderate activity was observed in the extracts of Cassia obtusifolia and Glyeine max var. chungtae, and C. lineata and Vigna angulasis against SK-MEL-2 and HCT-15 cell lines, respectively. The other seed extracts were ineffective against model tumor cell lines. Because of their potent cytotoxic activities, the activity of each solvent fraction from C. lineata and G. soja was determined and the potent activity was produced from their chloroform fractions. As a naturally occurring therapeutic agent, leguminous seeds described could be useful for developing new types of anti-tumor agents.

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