Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Cytotoxic Activity of Bombyx mori and Morus alba Derived Materials against Human Tumor Cell Lines

누에 및 뽕나무 유래 물질의 인간(人間) 암세포주(癌細胞株)에 대한 세포독성(細胞毒性)

  • Park, Il-Kwon (Division of Applied Biology and Chemistry, and the Research Center for New Bio-Materials in Agriculture, College of Agriculture and Life Sciences, Seoul National University) ;
  • Lee, Jeong-Ock (Korea Research Institute of Chemical Technology) ;
  • Lee, Hoi-Seon (Division of Applied Biology and Chemistry, and the Research Center for New Bio-Materials in Agriculture, College of Agriculture and Life Sciences, Seoul National University) ;
  • Seol, Kwang-Youl (National Institute of Sericulture and Entomology, Rural Development of Administration) ;
  • Ahn, Young-Joon (Division of Applied Biology and Chemistry, and the Research Center for New Bio-Materials in Agriculture, College of Agriculture and Life Sciences, Seoul National University)
  • 박일권 (서울대 응용생물화학부 및 농업생물신소재연구센터) ;
  • 이정옥 (한국화학연구소) ;
  • 이회선 (서울대 응용생물화학부 및 농업생물신소재연구센터) ;
  • 설광열 (농촌진흥청 잠사곤충연구소) ;
  • 안용준 (서울대 응용생물화학부 및 농업생물신소재연구센터)
  • Published : 1998.04.30
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Abstract

The cytotoxic activity of MeOH extracts of the freeze-dried silkworm (Bombyx mori)-derived materials (4th instar larvae, female and mate pupae, virgin female and male adult), dried Beauveria bassiana-infected silkworm larvae, dried feces from the 4th instar larvae B. mori, and dried mulberry (Morus alba)-derived materials (leaves, fruits, root barks) in vitro was evaluated by sulforhodamine B assay, using the five human solid A 549 lung, SK-OV-2 ovarian, SK-MEL-2 melanoma, XF-498 CNS and HCT-15 colon tumor cell lines. The responses varied with both cell line and material used. The 70% hot MeOH extract of B. mori feces (BFH) revealed potent cytotoxic activity against model tumor cell lines whereas moderate activity was observed from the MeOH extract of B. mori feces. M. alba root barks, and M. alba fruits. The other test materials were ineffective. Because of its potent cytotoxic activity, the activity of each solvent fraction from the BFH was determined. Chloroform and ethyl acetate fractions showed the most potent cytotoxic activity. In conclusion, our results may be an indication of at least one of the pharmacological actions of B. mori feces. M. alba root barks, and M. alba fruits.

동결건조(凍結乾燥)한 누에 유래(4령유충(齡幼蟲), 암 수 번데기, 암 수 성충(成蟲)) 및 건조 뽕나무 유래 재료(잎, 오디, 상백피(桑白皮))의 메탄을 추출물, 백강잠(白彊蠶) 및 누에 4령유충(齡幼蟲) 잠분(蠶糞)의 메탄올 추출물의 5종 인간(人間) 암세포주(癌細胞株)(A549 lung, SK-OV-2 ovarian, SK-MEL-2 melanoma, XF-498 CNS, HCT-15 colon tumor cell lines)에 대한 세포독성(細胞毒性)을 sulforhodamine B법을 이용하여 in vitro 검정하였다. 공시시료중 잠분(蠶糞)의 70% 메탄올 열탕추출물(熱湯抽出物)은 이들 암세포주(癌細胞株)에 대하여 강한 세포독성(細胞毒性)을 나타내었으나, 잠분(蠶糞)의 메탄올 추출물 및 오디와 상백피(桑白皮)의 메탄을 추출물은 중간 정도의 활성을 보였다. 기타 물질들은 이들 암세포주(癌細胞株)에 거의 독성을 보이지 않았다. 70% 메탄올 열탕추출물(熱湯抽出物)이 강한 세포독성(細胞毒性)을 나타내어, 용매 분획한 결과 클로로포름과 에틸아세테이트 획분이 암세포주(癌細胞株)에 대하여 가장 강한 세포독성(細胞毒性)을 보였다. 결론적으로, 잠분(蠶糞), 상백피(桑白皮) 및 오디의 항암활성(抗癌活性)은 이들의 약리작용의 일부를 설명할 수 있을 것으로 생각된다.

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