한국식품과학회지 (Korean Journal of Food Science and Technology)
- 제30권4호
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- Pages.976-982
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- 1998
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- 0367-6293(pISSN)
고사리 단백다당(Pteridium aquilinum Glycoprotein, PAG)이 마우스 면역활성에 미치는 영향
Effects of the Glycoprotein Isolated from Pteridium aquilinum on the Immune Function of Mice
- Park, Hyeon-Ae (Graduate School of Biotechnology, Korea University) ;
- Kweon, Mee-Hyang (Institute of Biotechnology, Korea University) ;
- Han, Hyung-Mee (Korea Food and Drug Administration) ;
- Sung, Ha-Chin (Graduate School of Biotechnology, Korea University) ;
- Yang, Han-Chul (Graduate School of Biotechnology, Korea University)
- 발행 : 1998.08.01
초록
고사리 수추출물에서 분리된 단백다당획분(Pteridium aquilinum glycoprotein, PAG)이 마우스 면역계에 미치는 영향을 알아보기 위하여 BALB/C 마우스의 복강내에 14일간 반복 투여한 후 마우스의 체중 및 면역장기의 무게변화를 관찰하면서 마우스 항체생성능과 비장세포 증식능에 대하여 조사하였다. PAG를 용량별
The effects of the glycoprotein (PAG) isolated from Pteridium aquilinum on the immune function was examined in mice. PAG was intraperitoneally administered into BALB/C mice for 14 days and the antibody forming ability to hen egg lysozyme (HEL) and the blastogenic responses of splenocytes were measured. PAG treatment significantly increased antibody formation to HEL in a dose-dependent manner. Blatogenesis of splenocytes in response to lipopolysaccharide (LPS, B-cell specific mitogen) or phytohemagglutinin (PHA, T-cell specific mitogen) was also increased after treatment with PAG, indicating that the PAG increases both humoral and cellular immunities. To examine whether the immune function of PAG was via a direct effect on the lymphocytes, splenocytes were isolated from BALB/C mice, exposed to various concentrations of PAG in vitro and the blastogenic responses were measured. In vitro exposure to PAG significantly increased blastogenesis of splenocytes to LPS up to