한국식품과학회지 (Korean Journal of Food Science and Technology)
- 제30권4호
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- Pages.775-780
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- 1998
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- 0367-6293(pISSN)
감마선조사 쇠고기의 유전독성 및 급성독성학적 안전성평가
Genotoxicological and Acute Toxicological Safeties of Gamma Irradiated Beef
- 강일준 (한림대학교 식품영양학과) ;
- 곽희진 (한림대학교 식품영양학과) ;
- 이병훈 (원광대학교 약학대학) ;
- 김광훈 (그린피아기술 주식회사) ;
- 변명우 (한국원자력연구소) ;
- 육홍선 (한국원자력연구소)
- Kang, Il-Jun (Department of Food Science and Nutrition, Hallym University) ;
- Kwak, Hee-Jin (Department of Food Science and Nutrition, Hallym University) ;
- Lee, Byung-Hoon (College of Pharmacy, Wonkwang University) ;
- Kim, Kwang-Hoon (Greenpia Tech Inc.) ;
- Byun, Myung-Woo (Korea Atomic Energy Research Institute) ;
- Yook, Hong-Sun (Korea Atomic Energy Research Institute)
- 발행 : 1998.08.01
초록
우육의 위생화를 위한 방사선 조사기술의 이용 가능성을 검토할 목적으로 방사선 조사 쇠고기(5 kGy)를 대상으로 유전독성 및 급성독성 시험을 통한 안전성 평가를 실시하였다. 감마선 조사 및 비조사 쇠고기 현탁액의 S. typhimurium TA98, TA100, TA1535 및 TA1537에 대한 복귀변이 집락수를 조사한 결과, 대사활성계 도입 및 부재시 모두, 모든 시험균주에서 시험 적용 농도인
Gamma irradiation at 5 kGy was applied to beefs for evaluation of their possible genotoxicity and acute oral toxicity. The genotoxicity of 5 kGy irradiated beef was evaluated by Salmonella typhimurium reversion assay and in vivo micronucleus assay using mouse bone marrow cells. The results were negative in the bacterial reversion assay with S. typhimurium TA98, TA100, TA1535, TA1537. Clastogenic effects were not shown in vivo mouse micronucleus assay at 5 kGy dose tested. In an acute toxicity test, 5 kGy-irradiated beef was administrated orally at a dose level of 313 to 5,000 mg/kg, and then number of deaths, clinical signs, body weights, and pathological examinations were examined daily for 14 days post-administration. The results indicate that 5 kGy irradiated beef did not show any toxic effect on mice and oral