한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
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SOS Chromotest에서 숙지황 물 추출물의 세포내 항돌연변이 효과

Bio-antimutagenic effects of water extract from Rehmannia glutinosa Liboschitz in SOS Chromotest

  • 안병용 (원광대학교 생명자원과학부) ;
  • 이갑상 (원광대학교 생명자원과학부) ;
  • 맹일경 (전북대학교 식품공학과) ;
  • 송근섭 (이리농공전문대학 식품공업과) ;
  • 최동성 (우석대학교 생물식품 및 환경화공학부)
  • Ahn, Byung-Yong (Division of Life Science and Natural Resource, Wonkwang University) ;
  • Lee, Kap-Sang (Division of Life Science and Natural Resource, Wonkwang University) ;
  • Maeng, Il-Kyung (Department of Food Science and Technology, Chonbuk National University) ;
  • Song, Geun-Seoub (Department of Food Engineering, Iri National College of Agriculure & Technology) ;
  • Choi, Dong-Seong (Division of Food, Enviromental and Chemical engineering and Biotechnology, Woosuk University)
  • 발행 : 1998.04.01
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초록

숙지황 물 추출물의 항돌연변이 활성을 4-nitroquinoline 1-oxide (4-NQO), N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), mitomycin C (MMC), $aflatoxin\;B_1\;(AFB_1)$, benzo(a)pyrene [B(a)P]의 변이원성에 대하여 SOS Chromotest로 조사하였다. 숙지황 물 추출물을 메탄올 가용성 부분과 불용성 부분으로 분리하여 시험한 결과 5종의 변이원의 활성에 대하여 메탄올 가용성 분획은 불용성 분획 보다 강한 억제효과를 나타내었다. 따라서 메탄올 가용성 분획을 에틸아세테이트, 부탄올, 물 분획 순으로 순차용매 분리하였고 그 분획물 중, 물 분획이 5종 변이원의 돌연변이원성에 대하여 가장 강한 억제효과$(4.5{\sim}29.5%)$를 나타내었고, 에틸아세테이트 분획은 $AFB_1$의 변이원성을 촉진하였다. Sephadex LH-20 column chromatography를 이용하여 물 분획을 정제하여 9 분획을 얻었으며, 이들 중 fraction III가 시험한 5종의 변이원으로 각각 유도된 변이원성에 대하여 용량반응의 억제효과와 함께 가장 강한 억제활성을 나타내었다. $400\;{\mu}g/assay$ 농도의 Fraction III는 4-NQO, MNNG, MMC, AFB1 및 B(a)P의 돌연변이원성에 대하여 각각 29, 35, 38, 25, 24% 억제효과를 나타내었다. Fraction III는 4-NQO, AFB1의 변이원성에 대하여 40% 이상의 강한 세포내 항돌연변이원성을 나타내었다.

The antimutagenic activity of the water extract of Rehmannia glutinosa Liboschitz (RG) on the mutagenicity induced by 4-nitroquinoline 1-oxide (4-NQO), N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), mitomycin C (MMC), $aflatoxin\;B_1\;(AFB_1)$ and benzo(a)pyrene [B(a)P] were studied using the SOS Chromotest with Escherichia coli PQ37. The water extract of RG was separated into methanol soluble and methanol insoluble parts. The methanol soluble part exhibited higher inhibition effects than the methanol insoluble part against the mutagenic activities of five mutagens. Step-wise fractionation of methanol soluble part was done using methanol, ethyl acetate and water. Among these fractions, water fraction had the strongest inhibitory effects against the mutagenenicity of five model mutagens, showing $4.5{\sim}29.5%$ inhibition, but the $AFB_1$ mutagenic potency was increased slightly by ethyl acetate fraction. The water fraction was further partitioned by sephadex LH-20 column chromtography, and 9 subfractions were obtained. The fraction III showed the strongest inhibitory effects with dose response against the mutagenic activities induced by all the tested chemical mutagens. The inhibition rates of fraction III at concentration of $400\;{\mu}g/assay$ were 29%, 35%, 38%, 25% and 24% against 4-NQO, MNNG, MMC, AFBl and B(a)P, respectively. The fraction III also exhibited a strong bio-an-timutagenicity against 4-NQO and $AFB_1$ by showing more than 40% inhibition.

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