Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Effect of Buthus martensi Karsch on Natural Killer Cell Activity in Mice

전갈(Buthus martensi Karsch)이 마우스 Neutral Killer 세포활성에 미치는 영향

  • Lee, Won-Hoon (Dept. of Internal Midicine, College of Oriental Medicine, Dongguk University) ;
  • Jeong, Ji-Cheon (Dept. of Internal Midicine, College of Oriental Medicine, Dongguk University) ;
  • Kim, Jong-Dae (Dept. of Internal Midicine, College of Oriental Medicine, Dongguk University) ;
  • Yoon, Cheorl-Ho (Dept. of Internal Midicine, College of Oriental Medicine, Dongguk University) ;
  • Seo, Woon-Gyo (Dept. of Internal Midicine, College of Oriental Medicine, Dongguk University) ;
  • Shin, Hyun-Chul (Dept. of Internal Midicine, College of Oriental Medicine, Dongguk University) ;
  • Lee, Dong-Mok (Dept. of Animal Science, College of Natural Resources, Taegu University) ;
  • Song, Hai-Bum (Dept. of Animal Science, College of Natural Resources, Taegu University) ;
  • Lee, Hang-Woo (College of Science, Yeungnam University) ;
  • Nam, Kyung-Soo (Dept. of Pharmacology, College of Medicine, Dongguk University)
  • 이원훈 (동국대학교 한의과대학 내과학교실) ;
  • 정지천 (동국대학교 한의과대학 내과학교실) ;
  • 김종대 (동국대학교 한의과대학 내과학교실) ;
  • 윤철호 (동국대학교 한의과대학 내과학교실) ;
  • 서운교 (동국대학교 한의과대학 내과학교실) ;
  • 신현철 (동국대학교 한의과대학 내과학교실) ;
  • 이동목 (대구대학교 자연자원대학 축산학과) ;
  • 송해범 (대구대학교 자연자원대학 축산학과) ;
  • 이항우 (영남대학교 이과대학 생물학과) ;
  • 남경수 (동국대학교 의과대학 약리학교실)
  • Published : 1998.12.30
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Abstract

The effects of Buthus martensi Karsch (BMK) on natural killer (NK) cell activity in mouse spleen were studied. Water extracted solution of BMK was orally administrated to Balb/c mice for 2 weeks. Among splenic cells, T cell fractions were separated by Nylon wool column. Furthermore, NK cell purification was performed 4.5% percoll gradients methods. The cytotoxcity of NK cell to K562 cell was determined by lactic acid dehydrogenase and $[^3H]-thymidine $ incorporation methods. And the cytotoxicity of effector cell was most effectively induced in a ration of 50:1 (effector/target cell). As a result, cytotoxicity of NK cells was significantly increased compared with control group both in vivo and in vitro systems. The similar cytotoxic effect was shown in $[^3H]-thymidine $ incorporation methods. This suggests that when BMK is administrated to mice with malignant tumors, an increase in NK cell activity may occur and affect K562 tumor cells.

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