The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Identification of Varieties by Biochemical Methods in Pleurotus spp.

느타리 버섯류(Pleurotus spp.)의 생화학적 방법에 의한 품종구분

  • Kim, Dong-Hyun (Natural Resources Graduate School, Korea Univ.) ;
  • Kong, Won-Sik (National Institute of Agricultural Science & Technology, RDA) ;
  • Kim, Kyung-Soo (National Institute of Agricultural Science & Technology, RDA) ;
  • Kim, Young-Ho (National Institute of Agricultural Science & Technology, RDA) ;
  • You, Chang-Hyun (National Institute of Agricultural Science & Technology, RDA) ;
  • Kim, Young-Bae (Natural Resources Graduate School, Korea Univ.)
  • 김동현 (고려대학교 자연자원대학원 식품공학과) ;
  • 공원식 (농업과학기술원 응용미생물과) ;
  • 김경수 (농업과학기술원 응용미생물과) ;
  • 김영호 (농업과학기술원 응용미생물과) ;
  • 유창현 (농업과학기술원 응용미생물과) ;
  • 김영배 (고려대학교 자연자원대학원 식품공학과)
  • Published : 1998.06.30
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Abstract

To identify genetic difference of 13 strains in three Pleurotus species, analyses of rDNA, AP-PCR and RFLP were carried out. IGRI and $ITSI{\sim}II$ regions of rDNA amplified by PCR were about 0.9 and 0.7 kb, respectively. These PCR products were digested with six restriction enzymes to analyse polymorphism. Especially, treatment of HaeIII enzyme on $ITSI{\sim}II$ regions showed specific bands in three Pleurotus sajor-caju strains. Genetic differences among three species were classified by similarity analyses based on rDNA polymorphism. Various band patterns of $2,500{\sim}150\;bp$ were showed by AP-PCR. Identification of species and varieties in 13 Pleurotus strains was possible according to primers used in AP-PCR. In order to develop genetic markers, RFLPs using IGRI and $ITSI{\sim}II$ probes derived from ASI 2180 and 2070 were carried out on eight Pleurotus varieties. RFLP patterns using IGRI probe were more various than that of $ITSI{\sim}II$ probe.

현재 우리나라에서 가장 많이 재배되고 있는 느타리버섯류 중 P. ostreatus, P. florida, P. sajorcaju의 3개 종 13개 품종에 대하여 rDNA분석 및 AP-PCR, RFLP를 실시하여 각 종 및 품종들에 대한 구분을 시도하였다. rDNA의 IGRI부위는 약0.9 kb로 증폭되었고, $ITSI{\sim}II$는 약 0.7 kb로 증폭되었다. 각 PCR 산물을 6가지 제한효소로 절단하여 polymorphism을 분석한 결과, $ITSI{\sim}II$ 부위를 HaeIII로 처리시 여름느타리에 특이적인 band를 보였다. 또한 유연관계를 분석하여 종간 차이를 구분할 수 있었다. AP-PCR를 실시한 결과 약 $2.0kb{\sim}150\;bp$의 다양한 band를 볼 수 있었고 P. florida종은 marker로 사용 가능한 특이 밴드가 발견되었다. 또한 사용된 primer에 따라 종간의 구별이 가능하였을 뿐 아니라 품종간에도 차이를 보이는 primer도 찾을 수 있었다. 품종을 구분하기 위한 RFLP 분석에서는 $ITSI{\sim}II$보다 IGRI probe가 더 큰 변이를 보였다.

Keywords

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