The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Effect of Bromocriptine on 6-Hydroxydopamine-induced Lipid Peroxidation and Cytotoxicity in vitro and in vivo

  • Kim, Yong-Sik (Department of Pharmacology, College of Medicine and Neuroscience Research Institute, Medical Research Center, Seoul National University) ;
  • Maeng, Sung-Ho (Department of Pharmacology, College of Medicine and Neuroscience Research Institute, Medical Research Center, Seoul National University) ;
  • Park, Chan-Woong (Department of Pharmacology, College of Medicine and Neuroscience Research Institute, Medical Research Center, Seoul National University)
  • Published : 1998.10.21
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Abstract

The present study was to evaluate the protective effects of bromocriptine, which is known as $D_2$ dopamine receptor agonist and used for the treatment of patients with Parkinson's disease (PD), on 6-hydroxydopamine (6-OHDA)-induced neurotoxicity in vitro and in vivo. Lipid peroxidation product (malondialdehyde; MDA) produced by the administration of 6-OHDA was profoundly reduced following the treatment of bromocriptine in a dose-dependent manner in rabbit brain homogenate. Quinone formation by 6-OHDA autoxidation was also attenuated, and its effect was as potent as other antioxidants. Pretreatment of bromocriptine reduced the cytotoxicity of 6-OHDA on SH-SY5Y neuroblastoma cell lines dose-dependently. The loss of striatal dopamine and its metabolite, DOPAC (dihydroxyphenylacetic acid) as well as increase of MDA production caused by intrastriatal injection of 6-OHDA was significantly recovered following the treatment of bromocriptine. The present study clearly showed that bromocriptine had a protective action against 6-OHDA-induced neurotoxicity. These results suggest that bromocriptine has the antioxidant properties, which could be another advantage for delaying the progress of Parkinson's disease.

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