Journal of Dairy Science and Biotechnology

Korean Society of Dairy Science and Biotechnology (한국낙농식품응용생물학회)
권호 표지

Screening and Partial Purification of Bacteriocins by Strains of Lactobacillus acidophilus Isolated from Human Origin

인체에서 분리된 Lactobacillus acidophilus가 생산하는 박테리오신의 선별과 정제

  • Kim, Se-Heon (Dept. of Animal Science, Korea University) ;
  • Kim, Yeong-Gyo (Dept. of Animal Science, Korea University) ;
  • Gilliland, S.E. (Dept. of Animal Science, Oklahoma State University)
  • 김세헌 (고려대학교 응용동물과학과) ;
  • 김영교 (고려대학교 응용동물과학과) ;
  • Published : 1997.05.24
Download PDF
⟨ Previous Next ⟩

Abstract

Lactobacillus acidophilus 223, 606, and NCFM-F among 21 isolated from fecal contents of humans demonstrated inhibitory activity attributed to bacteriocin(s). The bacteriocin(s) were heat stable and nondialyzable proteinous compounds and exhibited narrow inhibitory spectra of activity. Neither hydrogen peroxide nor pH were responsible for inhibitory action. All of the producer strains were resistant to their own bacteriocin(s). The bacteriocin(s) were purified by ammonium sulfate precipitation, gel chromatography and ion exchange chromatography for further characterization. The bacteriocin(s) of human origin exhibited similar characteristics.

Keywords