Effect of Antipsychotic Drugs on Dopamine Transporter Function in CV(bDAT) Cells

도파민 발현 세포주 CV(bDAT) 세포에서 항정신병 약물이 도파민 수송체 활성에 미치는 영향

  • Kim, Young-Mi (Department of Biochemistry, College of Medicine, Hanyang University) ;
  • Lee, Yong-Sung (Department of Biochemistry, College of Medicine, Hanyang University) ;
  • Lee, Sang-Hun (Department of Biochemistry, College of Medicine, Hanyang University) ;
  • Oh, Dong-Yul (National Seoul Mental Hospital) ;
  • Yang, Byung-Hwan (Department of Neuropsychiatry, College of Medicine & Mental Health Research Institute, Hanyang University) ;
  • Cho, Suck-Shin (Department of Nuclear Medicine, College of Medicine, Hanyang University) ;
  • Nam, Jung-Hyun (Department of Neuropsychiatry, College of Medicine & Mental Health Research Institute, Hanyang University)
  • 김영미 (한양대학교 의과대학 생화학교실) ;
  • 이용성 (한양대학교 의과대학 생화학교실) ;
  • 이상훈 (한양대학교 의과대학 생화학교실) ;
  • 오동렬 (국립서울정신병원) ;
  • 양병환 (한양대학교 정신건강연구소 및 의과대학 신경정신과학교실) ;
  • 조석신 (한양대학교 의과대학 핵의학교실) ;
  • 남정현 (한양대학교 정신건강연구소 및 의과대학 신경정신과학교실)
  • Published : 1997.06.25

Abstract

CV(bDAT) cell line, expressing dopamine transporter stably, has been established by transfection of CV-1 cells with bovine dopamine transporter cDNA. Using CV(bDAT) cells, the effects of various antipsychotic drugs on dopamine uptake activity were investigated. All of antipsychotic drugs tested, inhibited the [$^3H$]dopamine uptake into CV(bDAT) cells with $IC_{50}s$ in the low to mid micromolar range, implying that antipsychotic drugs may produce overflow of dopamine in the synaptic cleft of dopaminergic neuron.

Keywords