Korean Journal of Weed Science (한국잡초학회지)

The Korean Society of Weed Science & The Turfgrass Society of Korea Archive (한국잡초학회 & 한국잔디학회)
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Selective Mechanism of Cyhalofop-butyl ester between Rice(Oryzae sativa L.) and Echinochloa crus-galli - IV. Effect on Enzyme Activity, Biosynthesis of Fatty Acid and Protein in Rice and Echinochloa crus-galli

제초제(除草劑) Cyhalofop-butyl ester의 벼와 피간(間)의 선택성(選擇性) 기작(機作) - IV. 효소활성(酵素活性), 지방산(脂肪酸) 및 단백질합성(蛋白質合成)에 미치는 영향(影響)

  • Park, J.E. (National Institute of Agricultural Science & Technology, RDA.) ;
  • Lee, I.Y. (National Institute of Agricultural Science & Technology, RDA.) ;
  • Park, T.S. (National Institute of Agricultural Science & Technology, RDA.) ;
  • Ryu, G.H. (Development Administration) ;
  • Kim, Y.K. (National Institute of Agricultural Science & Technology, RDA.) ;
  • Kim, K.U. (Dept. of Agronomy, Kyungpook National university)
  • Received : 1997.04.02
  • Published : 1997.06.30
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Abstract

This experiment was carried out to determine selectivity of herbicide cyhalofop-butyl ester on enzyme activity, biosynthesis of fatty acid and protein between rice and Echinochloa crus-galli. Activity of the acetyl-CoA carboxylase(ACCase) was inhibited greater in E. crus-galli than in rice by the treatment of cyhalofop-butyl ester. The ACCase activity in E. crus-galli was observed with $I_{50}$ at 1-2ppm of cyhalofop-butyl ester, while in rice only at above 10ppm. Cyhalofop-butyl ester also inhibited the biosynthesis of fatty acid by 61% of palmitic acid, 54% of linoleic acid and 41% of linolenic acid in E. crux-galli. In contrast, no significant difference of fatty acid content was observed in rice at 5DAT as compared with the untreated control. Protein patterns of rice between the herbicide treatment and the untreated control were not significantly different, but in E. crus-galli, 3 protein spots were disappeared in between 29KD and 45KD.

벼와 피간(間)의 속간(屬間) 선택성(選擇性)을 구명(究明)하기 위하여 제초제(除草劑) cyhalofop-BE에 대(對)한 Acetyl-CoA carboxylase의 효소활성(酵素活性), 지방산(脂肪酸) 및 생합성(生合成) 단백질(蛋白質) 패턴에 미치는 영향(影響)을 시험(試驗)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. ACCase을 저해하는 농도는 피가 1-2ppm 농도(濃度)의 낮은 수준(水準)이었고 벼는 10배(倍) 이상(以上)의 높은 농도(濃度)에서 저해(沮害)되었다. 2 벼 및 피의 지방산(脂肪酸) 생합성(生合成)에 관여(關與)하는 ACCase의 효소(酵素)가 저해(沮害)되어 피는 포화지방산(飽和脂肪酸)인 palmitic acid가 무처리(無處理)에 비(比)해 61%, 불포화지방산(不飽和脂肪酸)인 linoleic acid가 54%, linolenic acid는 41%가 정도(程度) 억제(抑制)되었으나 벼는 무처리(無處理)와 큰 차이(差異)를 보이지 않았다. 3. 피는 29KD와 36KD사이 및 36KD 와 45KD사이의 spot가 소멸(消滅)되어 단백질(蛋白質)패턴에 영향(影響)이 있었다.

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