Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Chromosome Configurations of Human Oocytes Matured in vitro following Cryopreservation at the Germinal Vesicle Stage

인간 미성숙난자의 동결.융해후 체외 배양된 난자에 대한 염색체 분석

  • Park, S.E. (Infertility Medical Center, CHA General Hospital, College of Medicine, Pochon CHA University) ;
  • Chung, C.J. (Infertility Medical Center, CHA General Hospital, College of Medicine, Pochon CHA University) ;
  • Son, W.Y. (Infertility Medical Center, CHA General Hospital, College of Medicine, Pochon CHA University) ;
  • Chung, H.M. (Infertility Medical Center, CHA General Hospital, College of Medicine, Pochon CHA University) ;
  • Lee, S.H. (Infertility Medical Center, CHA General Hospital, College of Medicine, Pochon CHA University) ;
  • Lee, W.S. (Infertility Medical Center, CHA General Hospital, College of Medicine, Pochon CHA University) ;
  • Ko, J.J. (Infertility Medical Center, CHA General Hospital, College of Medicine, Pochon CHA University) ;
  • Yoon, T.K. (Infertility Medical Center, CHA General Hospital, College of Medicine, Pochon CHA University) ;
  • Cha, K.Y. (Infertility Medical Center, CHA General Hospital, College of Medicine, Pochon CHA University)
  • 박성은 (포천중문의과대학교 차병원 여성의학연구소) ;
  • 정창조 (포천중문의과대학교 차병원 여성의학연구소) ;
  • 손원영 (포천중문의과대학교 차병원 여성의학연구소) ;
  • 정형민 (포천중문의과대학교 차병원 여성의학연구소) ;
  • 이숙환 (포천중문의과대학교 차병원 여성의학연구소) ;
  • 이우식 (포천중문의과대학교 차병원 여성의학연구소) ;
  • 고정재 (포천중문의과대학교 차병원 여성의학연구소) ;
  • 윤태기 (포천중문의과대학교 차병원 여성의학연구소) ;
  • 차광열 (포천중문의과대학교 차병원 여성의학연구소)
  • Published : 1997.08.30
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Abstract

Objective: To investigate effects of cryoprotectant and cryopreservation on the chromosome of the human immature oocytes. Design: Intact cumulus-enclosed immature oocytes were collected from unstimulated ovaries and divided into three groups, such as no treatment as control (group 1), only 1,2-propanediol (PROH)-treated (group 2), and cryopreserved oocytes (group 3). Oocytes in group 1, 2, and survived oocytes after cryopreservation in group 3 were cultured for 48 hours. Setting: Infertility Medical Center at the CHA General Hospital, Seoul, Korea. Patients: Oocytes were obtained from Patients undergoing gynecological surgery. Main Outcome Measures: Maturation rate, abnormality in chromosomes by fluorescence in situ hybridization (FISH). Results: There was no effect of PROH only treatment on the chromosomal abnormalities in group 2 compared to control oocytes (41.4% and 31.8%, respectively). Whereas significantly increased abnormalities in chromosome (77.8%) were found in group 3. Conclusions: Human oocytes matured in vitro after cryopreservation at the germinal vesicle (GV) stage showed increased incidence of chromosomal abnormalities. These abnormalities may impair the capacity for further development of the embryos derived from frozen-thawed oocytes.

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