Clinical and Experimental Reproductive Medicine
- Volume 24 Issue 3
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- Pages.369-375
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- 1997
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
The Optimization of Human Sperm Decondensation Procedure for Fluorescence in Situ Hybridization
Fluorescence in Situ Hybridization 시행을 위한 인간정자 탈응축법의 적정화
- Pang, Myung-Geol (Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University)
- 방명걸 (서울대학교 의학연구원 인구의학연구소)
- Published : 1997.12.30
Abstract
Studies were conducted to determine the efficiency of decondensation protocols. Sperm obtained from seven normal donors was immediately washed after liquefaction and then decondensed using the method of West et al. (1989) and my original protocol. My optimized protocol entailed mixing 1 ml aliquots of semen with 4 ml phosphate buffered saline (PBS). Following centrifugation, pellets were resuspended in 1 ml PBS containing 6 mM EDTA. After centrifugation, pellets were resuspended in 1 ml PBS containing 2 mM dithiothreitol at