이간분수산(利肝分水散)이 Galactosamine에 의해 유발(誘發)된 백서(白鼠) 간기능손상(肝機能損傷)에 미치는 영향(影響)

Effect of Leegapunsusan on galactosamine induced hepatoxity in rats

이간분수산(利肝分水散)의 galactosamine에 의해 야기된 백서의 간손상에 대한 해독능을 측정하기 위하여 이간분수산(利肝分水散)을 전처지한 후 시험관내에서 과산화지질의 생성 및 간장의 지질과산화물의 함량 및 간장에서 항산화방지제(SOD)의 활성에 미치는 영향, 시험관내에서 xanthine oxidase의 활성변화 및 간장조직의 xanthine oxidase의 형전환 변화를 측정하여 유의한 결과를 나타내었는데 이는 이간분수산(利肝分水散)의 이요작용(利尿作用)을 통한 독성물질의 배설을 촉진시킴으로서 세포나 조직의 손상 및 생체독성에 대하여 유의한 방어효능을 나타내는 것으로 이해할 수 있다.

The purpose of this study is to observe tile protective effect of Leeganpunsusan on serum reaction and hepatic tissue in galactosamine treated rats. In this study, the experimental rats divided three group(Normal, Control and sample group). Under the same condition, normal and control groups were administered water, sample group was administered Leeganpunsusan for 15 days. The last day, both normal and control goups were injected to abdomen with galactosamine. The rates of lipid peroxide, SOD(activity) and xanthine oxidase(activity) were measured. The results were obtained as follows : Effects of the extract of Leeganpnnsusan on the hepatic lipid peroxidation, xanthine oxidase activity in VITRO, as compared with control group were significantly decreased with the level of concentration of extract prepared from Leeganpunsusan. In VIVO, the hepatic content of lipid peroxide, the rate of type changing(type D to O) and xanthine oxidase activity were significantly decreased in galactosamine-treated rats. Effect of on the hepatic cytosolic superoxide dismutase activity was significantly increased.