Restorative Dentistry and Endodontics
- Volume 22 Issue 2
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- Pages.693-701
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- 1997
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- 2234-7658(pISSN)
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- 2234-7666(eISSN)
DIFFERENTIATION OF PREVOTELLA INTERMEDIA AND P. NIGRESCENS USING SDS-PAGE
SDS-PAGE를 이 용한 Prevotella intermedia와 P. nigrescens의 감별에 관한 연구
- Bae, Kwang-Shik (Dept. of Conservative Dentistry, College of Dentistry, Seoul National University)
- 배광식 (서울대학교 치과대학 치과보존학교실)
- Published : 1997.09.30
Abstract
In 1992, Prevotella intermedia was shown to be comprised of another spoecies now known as Prevotella nigrescens. Strain ATCC 33563 is now designated the type strain of P. nigrescens while strain ATCC 25611 is remains the type strain of P. intermedia. The purpose of this study was to find the differences in protein profiles of P. intermedia and P. nigrescens, using sodium dodecyl sulfate polyacrylamide gel electrophoresis, which can be used for differentiation of those two species. A partial amino acid sequence of the 18.6 kDa protein band, which was specific in P. nigrescens, was also determined. The cellular proteins were extracted from the cell pellets of pure cultures of P. intermedia. and P. nigrescens by either sonication or being shaken continuously for 20 min at
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