Screening and Identification of Actinomycetes Producing Novel Elastase Inhibitor

Elastase Inhibitor 생성 방선균의 탐색 및 동정

Elastase inhibitor (EI) producing microorganisms were screened by microplate-assay of culture broth using Succinyl-$(Alanine)_3$-Nitroanilide as a chromogenic substrate. The portion of microorganisms showed EI activity above 90% was about 2% of all assayed. Isolated SMF-11 showed relatively high EI activity after filtration using membrane filter of NMWL 3,000. SMF-11 was identified as Streptomyces sp. according to results of morphological or physicochemical identification. SMF-11 has both rectiflexible and spiral spore chains with smooth spore surface and cell wall contained LL-DAP, iso- and anteiso-fatty acids. The amino acids in cell wall were alanine, glutamic acid and glycine. Fifty unit characters for major cluster were tested and the data were analyzed numerically using the TAXON program. The isolate SMF-11 was identified as a strain of Streptomyces lavendulae.

Elastase 저해물질(EI) 생성균주로 토양분리주 SMF-11을 선별하였다. 분리주 SMF-11는 매끈한 표면의 rectiflexible 및 spiral의 포자 사슬을 동시에 형성하고 세포벽 성분으로 LL-DAP, ios- 및 anteiso-fatty acid($C_{14}-C_{17}$), 세포벽 아미노산으로 alanine, glutamic acid, glycine을 가지고 있어 방선균속으로 분류되었다. 종 수준의 동정을 위하여 화학적 시험과 50가지의 단위형질에 대한 특성을 조사하고 TAXON program으로 수리분류한 결과, SMF-11 분리주는 방선균 주군집 61의 중심균주인 Streptomyces lavendulae로 동정하였다.