Quizalofop-Ethyl이 콩과 옥수수의 Glutathione-S-Transferases와 Carboxylesterase의 활성에 미치는 영향

Effect of Quizalofop-Ethyl on Glutathione-S-Transferases and Carboxylesterase Activity of Soybean and Corn Plants

콩과 옥수수에서 glutatione-S-transferases와 carboxylesterase의 생화학적 특성과 quizalofopethyl 처리에 따른 식물체내 효소의 활성 변화를 조사한 결과는 다음과 같다. GSTS의 최적 pH는 8.2이고, Km값은 콩 $6.7{\times}10^{-3}M$, 옥수수 $2.5{\times}10^{-3}M$이었으며, Vmax는 콩 50nmole/mg/min, 옥수수 20nmole/mg/min이었다. Carboxylesterase의 최적 pH는 6.2${\sim}$7.0 범위이고, Km값은 콩 $4.2{\times}10^{-4}M$, 옥수수 $2.5{\times}10^{-4}M$이었으며, Vmax는 콩 33 nmole/mg/min, 옥수수 10nmole/mg/min이었다. 콩과 옥수수에 살포된 quizalofop-ethyl은 콩과 옥수수의 GSTs와 carboxy-lesterase의 활성을 감소시켰고, quizalofop-ethyl 80ppm을 처리했을때 콩은 10일이 경과후 회복되었지만, 옥수수는 3일이 경과후 고사하였다.

Biochemical characteristics and activities of glutathione-S-transferases(GSTs) and carboxylesterase extracted from soybean and corn plants treated with quizalofop-ethyl were investigated. Km value and Vmax of GSTs extracted from soybean and corn plants were $6.7{\times}10^{-3}M$ nmole/mg/min, 50, 20 nmole/mg/min, respectively. Optimum pH of carboxylesterase from soybean and corn was 7.0. Km value and Vmax of carboxylesterase extracted from soybean and corn plants were $4.2{\times}10^{-4}M$, $2.5{\times}10^{-4}M$ nmole/mg/min, 33, 10 nmole/mg/min, respectively. GSTs and carboxylesterase activity were reduced by quizalofop-ethyl. GSTs and carboxylesterse activity of corn was more reduced than that of soybean. When soybean and corn were treated by 80 ppm of quizalofopethyl. Soybean recovered after 10 days elapsing, but corn withered after 3days elapsing.