Korean Journal of Environmental Agriculture (한국환경농학회지)
- Volume 16 Issue 1
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- Pages.37-43
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- 1997
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- 1225-3537(pISSN)
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- 2233-4173(eISSN)
Tolerance Mechanism to Simazine in Coix lacryma-jobi
율무(Coix lacryma-jobi)의 제초제 Simazine에 대한 내성기구
- Ma, Sang-Yong (Division of Life Resource Science, Woosuk University) ;
- Kim, Jong-Seok (Division of Life Resource Science, Woosuk University) ;
- Chun, Jae-Chul (Dept. of Agricultural Chemistry, Jeonbuk National University)
- Published : 1997.03.31
Abstract
Tolerance mechanism to simazine (6-chloro-N,N'-diethyl-1,3,5-triazine-2,4-diamine) in Coix lacryma-jobi was investigated with respect to herbicide detoxification via glutathione conjugation. Simazine was initially absorbed by seedlings of C. lacryma-jobi and corn, but after 12 hours of treatment, no significant difference in simazine absorption was found in both species. Simazine absorbed was rapidly metabolized to glutathione-simazine conjugate. One to six hours after treatment, metabolism was approximately 2-fold faster in C. lacryma-jobi than in corn. Glutathione content was found 1.5- and 2.3-fold higher in coleoptile and root of C. lacryma-jobi, respectively, compared with corn. In both species, the highest concentration of glutathione was found in coleoptile tissue. Glutathione S-transferase that exhibits activity with 1-chloro-2,4-dinitrobenzene was not significantly different between two species. However, glutathione S-transferase activity with simazine was approximately 2-fold greater in C. lacryma-jobi than in corn. The glutathione S-transferase activity was 20 to 30% greater in shoot of either species than in root. Fast protein liquid chromatography-anion exchange column was used to separate glutathione S-transferase isozymes in coleoptiles of C. lacryma-jobi and corn. A peak of glutathione S-transferase activity with 1-chloro-2,4-dinitrobenzene and two peaks of glutathione S-transferase activity with simazine from C. lacryma-jobi were coeluted with those from corn, but showed greater activity than in the case of corn. Another glutathione S-transferase isozyme that exhibits activity with simazine was detected in the elution of C. lacryma-jobi extract, but not in corn. Electron transport in chloroplast thylakoids isolated from leaves of both species was equally sensitive to simazine applied at 1 to 100 nM. These results indicate that the simazine tolerance in C. lacryma-jobi is due to its capacity to detoxify the herbicide via glutathione conjugation, which is positively correlated with the level of glutathione content and glutathione S-transferase activity.
율무의 simazine에 대한 내성이 GSH와의 conjugation에 의한 약제의 불활성화 반응에 기인하는지 여부를 검정하기 위하여 화본과 작물인 옥수수와 비교하여 약제의 흡수 및 대사속도, GSH 함량 및 GST 활성, GST isozyme의 발현 양상 및 작용점에서의 약제에 대한 감수성의 차이를 조사하였다. 율무와 옥수수 모두 배양액중에 포함된 simazine에 대하여 약제처리 초기부터 빠른 흡수를 보였으며, 처리 12시간 후에는 두 초종 간의 흡수속도에 있어서 차이가 인정되지 않았다. 율무에 흡수된 simazine은 GSH-simazine conjugate의 형태로 신속히 대사되었으며, 특히 약제처리
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