Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
권호 표지

Biological Control of Phytophthora Blight of Red-pepper Caused by Phytophthora capsici.;Ⅲ. Identification of the Antifungal Substances Produced by Pseudomonas sp. A - 183.

고추역병균(疫病菌)(Phytophthora capsici)의 생물학적(生物學的) 방제(防除);Ⅲ. 항균물질(抗菌物質)의 구조분석(構造分析)

  • Chang, Yoon-Hee (Department of Agricultural Chemistry, College of Agriculture, Kyungpook National University) ;
  • Jang, Sang-Moon (Department of Agricultural Chemistry, College of Agriculture, Kyungpook National University) ;
  • Choi, Jyung (Department of Agricultural Chemistry, College of Agriculture, Kyungpook National University) ;
  • Lee, Dong-Hoon (Department of Agricultural Chemistry, College of Agriculture, Kyungpook National University)
  • 장윤희 (경북대학교 농과대학 농화학과) ;
  • 장상문 (경북대학교 농과대학 농화학과) ;
  • 최정 (경북대학교 농과대학 농화학과) ;
  • 이동훈 (경북대학교 농과대학 농화학과)
  • Published : 1997.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to identify the three antifungal substances isolated from the culture medium of Pseudomonas sp. A-183 which is antagonistic against Phytophthora capsici. The substance A and B showed positive reactions at the Molish test and Anthrone test, but negative one at the Fehling test, strongly suggesting that both substance A and B had nonreducing sugar frameworks. The substance C only exhibited the phenomenon of the UV induced fluorescence. From the qualitative analysis with the spectroscopic techniques such as UV, Mass, IR and NMR, the substance A and B were known to be composed to sugar and fatty acid, and showed a base peak of 171(m/e). It was identified that substance A was $(2-O-L-rhamnosyl-{\alpha}-L-rhamnosyl-{\beta}-hydroxydecanoyl-{\beta}-hydroxy$ decanoic acid) and the substance B was $({\alpha}-L-rhamnosyl-{\beta}-hydroxydecanoyl-{\beta}-hydroxy$ decanoic acid). The substance C was identified as a phenazine from the results of qualitative analysis with the spectroscopic techniques such as UV, Mass, IR and NMR.

고추 역병균(疫病菌)(P. capsici)에 우수(優秀)한 항균활성(抗菌活性)을 가진 3가지의 항균물질(抗菌物質)을 Pseudomonas sp. A-183 배양액(培養液)으로 부터 분리(分離) 정제(精製)하였다. 분리(分離)된 3가지의 항균물질(抗菌物質)에 대한 분자구조식(分子構造式)을 구명(究明)하기 위하여 UV, IR, $^1H$ NMR, $^{13}C$ NMR Spectroscopy법(法)으로 해석하였다. A와 B물질(物質)은 Molish 및 Anthrone반응(反應)에 양성(陽性)으로, Fehling반응(反應)에는 음성(陰性)으로 나타났으므로 nonreducing sugar구조(構造)를 함유(含有)하고 있는 것으로 판단되었다. C물질(物質)은 위의 시약(試藥)에서 정색반응(呈色反應)을 나타내지 않고 단지 UV흡수(吸收)만을 나타내었다. Mass, NMR 및 IR 분광분석(分光分析)에 의해 A와 B물질(物質)은 당(糖)으로는 deoxyhexose인 rhamnose를 포함하고 지방산으로는 ${\beta}-hydroxydecanoic$ acid를 포함하였다. 또 이들 사이에는 O-glycoside결합(結合)이 형성(形成)되어 있었으며 A와 B물질(物質) 사이에는 당(糖) 한분자(分子)의 차리(差異)가 나타났다. 종합적(綜合的)으로 A물질(物質)은 glycolipid B인 3-hydroxy-(3-hydroxydecanoyl) -decanoic acid $3-O-{\alpha}-L-rhamnopyranosyl(1{\to}2)rhamnopyranoside$, B물질(物質)은 glycolipid A인 3-hydroxy-(3`-hydroxydecanoyl)decanoic acid $3-O-{\alpha}-L-rhamnopyranoside$로 동정(同定)되었으며, C물질(物質)은 phenazine으로 확인(確認)되었다.

Keywords