YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Production Conditions and Characterization of ${\beta}$-Lactamase Inhibitor from Pseudomonas sp. X-8

슈도모나스 sp. X-8의 베타락타마제 억제제의 생산 조건과 특성

  • 김경자 (순천향대학교 자연과학대학 생명과학부) ;
  • 김태성 (순천향대학교 자연과학대학 생명과학부)
  • Published : 1997.10.01
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Abstract

Identification of a soil microorganism strain X-8, producer of ${\beta}$-lactamase inhibitor, based on its morphological, physiological, biochemical and chemotaxonomical characteristics was performed. The strain X-8 was identified as Pseudomonas sp. The beta-lactamase inhibitor produced by this strain was highly achieved in fermentation medium contained glucose 0.5%, urea 0.25%, $K_2HPO_4{\cdot}3H_2O\;0.5%,\;MgSO_4{\cdot}7H_2O\;0.5%,\;FeSO_4{\cdot}7H_2O\;0.01%,\;CuSO_4,\;ZnSO_4,\;MnSO_4\;0.02%$. The beta-lactamase inhibitor was not extracted by organic solvent such as n-butanol and ethyl acetate but remained in aqueous layer. The n-butanol extract showed antimicrobial activity against M. smegmatis. The ${\beta}$-lactamase inhibitor was stable at pH 7.0~8.0 and 4$^{\circ}C$ for 24h. The ${\beta}$-lactamase inhibitor was bound on ion exchanger Diaion WA-30 and HP-20 and eluted with 2N-$NH_4OH$ and acetone, respectively.

Keywords

References

  1. Antimicrob. Agent. Chemother. v.11 Clavulanic Acid: A β-Lactam from Streptomyces Clavuligerus. Reading, C.;Cole, M.
  2. Recent Adbances in the Chemistry of β-lactam Antibioics. In Recent dvances in the Chemistry of β-Lactam Antibiotics. Ed. Woodward, R. B.
  3. Bacterial resistance to β-lactam antibiotics : an overview. In β-Lactam Antibiotics. Edited by M. R. Salton and G. D. Shockman. Sykes, R. B.;Gorgopapadakou, N. H.
  4. Biochem. J. v.211 Mechanism of Inactivition of TEM-1 β-Lactamase by 6-Acetylmethyl-enepenicillanic acid. Arisawa, M.;Adam, S.
  5. Biochem. J. v.107 Properties of β-lactamases produced by three species of mycobacteria. Kasik, J. E.;Peacham, L.
  6. Int. J. Clin. Pharmacol. v.3 The mechanism of mycobacterial resistance to penicillins and cephalosporin. Misra, K.;Kasik, J. E.
  7. FEMS Microbiol. Letters. v.25 Penicillinbinding proteins in Mycobacterium smegmatis. Yabu, K.;Ochiai, T.;Kaneda, S.
  8. Biochem. J. v.209 The inhibition of class C β-lacta-mases by boronic acids. Beeley, T. N.;Gascoyne, V. K.;Hunziker, S.;Petursson, S. G.;Waley, B.;Jaurin, B.;Grundstrom, T.
  9. J. Antibiot. XLⅢ. v.4 Characteristica of Aztreonam as a substrate, inhibitor and inducer for β-lactamases. Sakurai, Y.;Yoshida, Y.;Saitoh, K.;Nemoto, M.;Yamaguchi, M.;Sawai, M.
  10. J. Antibiot. v.48 no.10 Isolation and structure determination of two novel phenazines from a Streptomyces with inhibitory activity against metallo-enzymes, including metallo-β-lactamases. Gipin, M. L.;Fulston, M.;Payne, D.
  11. FEMS Microbiol. v.143 an additional ionic bond suggested by molecular modelling of TEM-2 might induce a slight discrepancy between catalytic properties of TEM-1 and TEM-2 β-lac-tamases. Chaibe, E. B.;Farzaneh, S.;Peduzzi, J.;Barthelemy, M.;Labia, R.
  12. Antimicrob. Agents Chemother. v.1 Novel method for detect- ion of β-lactamase by using a chromogenic cephalosporin substrate. O'Callahan, C. H.;Morris, A.;Kirby, S. M.;Shingler, A. H.
  13. IRCS Med. Sci. v.13 Lipase and Penicillinase activity in Mycobacterium Ieprae. Alati, S.;Mahadevan P. R.
  14. J. Antibiot. v.29 Naturally-occuring β-lactamase inhibitors with antibacterial activity. Brown, A. G.;Butterworth D.;Cole M.;Hanscomb G.;Hood, J. D.;Reading, C.;Rolinson, G. N.
  15. Antimicrob. Agents Chemother. v.14 CP-45,889, a β-lactamase inhibitor that extends the antibacterial spectrum of β-lactams : Inhibital bacteriological characterization. English, A. R.;Retsema, J. A.;Girard, A. E.;Lynch, J. E.;Barth, W. E.
  16. J. Antibiotics. v.30 Isolation and Structure of a β-Lactamase Inhibitor from Streptomyces. Miyamura, K.;Takahashi, S.;Sezaki, M.;Linuma, K.;Naganawa, H.;Kondo, S.;Ohno, M.;Umezawa, M.
  17. J. Antibiotics. v.25 Studies on Penicillinase Inhibitors Produced by Microorganism. Hata,T.;Qmura, S.;Iwai, Y.;Ohno, H.;Takeshima, H.;Yamaguch, N.
  18. Anal. Chem. v.57 Convenient Spectrophotometric Method for Determination of Clavulanic Acid. Gutman, A. R.;Ribon, V.;Leblance, J.;Rapid, P.
  19. Chemotheraphy. v.34 β-Lactamase Inhibition by Acetylene Penicillanic Acid Compared to that of Clavulanate and Sulbactam. Chin, N. X.;Mcelrath, M. J.;Neu, H. C.
  20. Biochem. v.17 no.11 Kinetic studies on the inactivation of Escherichia coli RTEM β-lactamases by clavulanic acid. Fisher, J.;Charnas, R. L.;Knowles, J. R.
  21. Antimicrob. Agents and Chemother. v.38 Comparative Activities of Clavulanic Acid, Sulbactam and Azobactam against Clinically Important β-Lactamases. Payne, D. J.;Cramp, R.;Winstanley, D.;Knowles, D. J.
  22. J. Antibiot. v.32 Olivanic Acids, A Family of β-Lactam Antibiotics with β-Lactamase Inhibitory Properties produced by Streptomyces species I.Detection, Properties and Fermemtation studies. Butterworth, D.;Cole, M.;Hanscomb, G.;Rolison, G. M.
  23. Biochem. J. v.253 Imipenem as substrate and inhibitor of β-lactamases. Monks, J.;Waley, S. G.
  24. J. Antibiot. v.32 Thienamycin, A new β-lactam antibitic I. Discovery, Taxonomy, Isolation and Physical properties. Kahan, K. S.;Kahan, F. M.;Goegelman, R.;Currie, S. A.;Jackson, M.;Stapley, E. O.;Miller, W.;Miller, A. K.;Hendlin, D.;Mochales, S.;Hernandez, S.;Woodruff, H. B.;Irnbaum, J.
  25. J. Antibiot. XLⅢ(1) 6-(substituted methylene)penems, potent broad spectrum inhibitors of bacterial β-lactamase. I. Racemic 6-ethylidenepenems. Basker, M. J.;Osborne, N. F.
  26. Biochemistry. v.20 Penicillanic Acid Sulfone : An Unexpected Isotope Effect in the Interaction of 6α-and 6β-Mono-deuterio and 6,6-Dideuterio Derivatives with RTEM β-Lactamase from Escherichia coli. Brenner, D. G.;Knowles, J. R.
  27. J. Antibiot. v.48 no.11 Effects of a β-lactamase inhibitor, Sulbactam, on the activity of carbapenems against Pseudomonas aeruginosa. Hanumi, N.;Kato, Y.;Hashizume, T.;Matsuda, K.
  28. J. Antibiot. v.44 no.8 (6R, 8S)-(2-benzimidazolyl)hydroxymethyl-penicillanic acid as potent antibacterial agents and β-lactamase inibitor. Chen, Y. L.;Hedeberg, K.;Guarino, K.
  29. v.144 Trancriptional analysis and heterologous expression of the gene encoding β-lactamase inhibitory protein from Strptomyces clavuligerus. Gene. Paradkar, A. S.;Petrich, A. K.;Leskiw, B. K.;Aidoo, K. A.;Jensen, S. E.
  30. J. Bacteriol. v.172 Isolation and characterization of a β-lactamase-inhibitory protein from Strptomyces clavuligerus and cloning and analysis of the corresponding gene. Doran, J. L.;Leskiw, B. K.;Aippersbach, S.;Jensen, S. E.
  31. J. Antibiot. v.32 Olivanic acids, A family of β-lac-tam antibiotics with β-lactamase inhibitory properties produced by Streptomyces species I. Detection, Properties and Fermentation studies. Butterworth, D.;Cole, M.;Hanscomb, G.;Rolinson, V.
  32. Streptomyces Type Culture Projector, Methods Manual. Shirling, E. B.;Gottlieb, D.
  33. Nature v.202 Selection of media for isolation of Streptomycetes. Kuster, E.;Williams, S. T.