YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Effect of Tumor Necrosis Factor-${\alpha}$(TNF) on the Expression of Oncogenes in ME-180 Human Cervical Carcinoma Cells

종양괴사인자(TNF)가 ME-180 사람 경부 암종세포에서 종양 발생 유전자의 발현에 미치는 영향

  • Han, Hyung-Mee (Immunotoxicology Division, Department of Toxicology, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Kim, Hyung-Soo (Immunotoxicology Division, Department of Toxicology, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Sohn, Kyung-Hee (Immunotoxicology Division, Department of Toxicology, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Choi, Kyoung-Baek (Immunotoxicology Division, Department of Toxicology, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Chung, Seung-Tae (Immunotoxicology Division, Department of Toxicology, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Kim, Jin-Ho (Immunotoxicology Division, Department of Toxicology, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Lee, Byung-Moo (College of Pharmacy, SungKyunKwan University) ;
  • Kim, Joo-Il (Immunotoxicology Division, Department of Toxicology, National Institute of Toxicological Research, Korea Food and Drug Administration)
  • 한형미 (식품의약품안전본부 독성부 면역독성과) ;
  • 김형수 (식품의약품안전본부 독성부 면역독성과) ;
  • 손경희 (식품의약품안전본부 독성부 면역독성과) ;
  • 최경백 (식품의약품안전본부 독성부 면역독성과) ;
  • 정승태 (식품의약품안전본부 독성부 면역독성과) ;
  • 김진호 (식품의약품안전본부 독성부 면역독성과) ;
  • 이병무 (성균관대학교 약학대학) ;
  • 김주일 (식품의약품안전본부 독성부 면역독성과)
  • Published : 1997.10.01
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Abstract

Tumor necrosis factor-${alpha}$ (TNF) induced a cytotoxic response in ME-180 cervical carcinoma cells in vitro. This cytotoxic response was accompanied by a temporal series of mitogenic stimuli : increased c-fos, c-jun and jun-B expression. Depletion of protein kinase C (PKC) by exposure of ME-180 cells to 100ng/ml phorbol myristate acetate (PMA) for 24hours almost completely abolished TNF-mediated increase in these signals, indicating that a PKC-dependent pathway is involved in TNF-mediated increases in the expression of c-fos, c-jun and jun-B. Characteristics of TNF receptors after exposure to 100ng/ml PMA or 24hours were not altered, suggesting that diminished induction of these oncogenes by TNF after PMA treatment is not due to any changes at the receptor level. To examine whether a PKC-dependent pathway is involved in TNF-mediated cytotoxicity in ME-180 cells, cytotoxicity was measured after depletion of PKC. No apparent changes in cytototoxicity after PKC depletion suggest that a PKC-dependent pathway is not involved in TNF-mediated cytotoxicity. Furthermore, results from cytotoxicity tests after exposure to staurosporine (PKC inhibitor) did not show any changes in the TNF-mediated cytotoxicity, confirming that a PKC-dependent pathway is not involved in this process. These data indicate that 1) TNF induces expression of c-fos, c-jun and jun-B oncogenes via a PKC-dependent pathway and 2) PKC-dependent expression of these three oncogenes by TNF may not be involved in TNF-mediated cytotoxicity in ME-180 cells.

Keywords

References

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