The Journal of Korean Society of Virology (대한바이러스학회지)
- Volume 27 Issue 2
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- Pages.209-216
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- 1997
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- 1225-2344(pISSN)
Analysis of the Three Dimensional Structure of Envelope Protein of the Japanes encephalitis virus Isolated in Korea
국내에서 분리된 일본뇌염 바이러스의 Envelope Protein의 3차구조 분석
- Nam, Jae-Hwan (Division of Arboviruses, Department of Viral disease, National Institute of Health) ;
- Chae, Soo-Lim (Division of Arboviruses, Department of Viral disease, National Institute of Health) ;
- Kim, Eung-Jung (Division of Arboviruses, Department of Viral disease, National Institute of Health) ;
- Yoon, Kyung-Sik (Division of Arboviruses, Department of Viral disease, National Institute of Health) ;
- Lee, Ho-Dong (Division of Arboviruses, Department of Viral disease, National Institute of Health) ;
- Koh, Hyun-Chul (Department of Pharmacology, College of Medicine, Hanyang University) ;
- Cho, Hae-Wol (Division of Arboviruses, Department of Viral disease, National Institute of Health)
- 남재환 (국립보건원 바이러스질환부 신경계바이러스과) ;
- 채수림 (국립보건원 바이러스질환부 신경계바이러스과) ;
- 김은정 (국립보건원 바이러스질환부 신경계바이러스과) ;
- 윤경식 (국립보건원 바이러스질환부 신경계바이러스과) ;
- 이호동 (국립보건원 바이러스질환부 신경계바이러스과) ;
- 고현철 (한양대학교 의과대학 약리학교실) ;
- 조해월 (국립보건원 바이러스질환부 신경계바이러스과)
- Published : 1997.12.30
Abstract
Three dimensional structures of envelope protein from Korean isolates and Nakayama-NIH strain of Japanese encephalitis virus (JEV) were deduced by a computer program (HyperChem 4.0 Chemplus 1.0) based on the data of the three dimentional structure of Tick-borne encephalitis virus. In the three dimensional structure of envelope protein, neutralizing epitope and T-helper cell recognition site of C-terminal region of Korean isolates were structually similar to those of Nakayama-NIH but the N-terminal region was not. Korean JE isolates were compared with Nakayama-NIH strain by using cross-neutralization antibody test. Neutralizing activities of Korean isolates derived from guinea pigs were higher than those of Nakayama-NIH strain against Korean isolates, although the polyclonal antibody titers of Nakayama-NlH showed 1:160 to 1:640 against Korean isolates. According to the results from three dimentional structures and cross-neutralization analyses, the antigenic difference between Korean JE isolates and Nakayama-NIH strain may be dependent on structural difference of envelope protein.
Keywords
- Japanese encephalitis virus;
- Three dimensional structure;
- Envelope protein;
- Cross-neutralization test