The Journal of Korean Society of Virology (대한바이러스학회지)
- Volume 27 Issue 2
- /
- Pages.115-128
- /
- 1997
- /
- 1225-2344(pISSN)
Cloning and Expression of NS5 Region of Korean Type Hepatitis C Virus
한국형 C형 간염 바이러스의 NS5 지역 cDNA 클로닝과 발현
- Han, Dong-Pyou (Department of Life Science, Sogang University) ;
- Lee, Taek-Youl (Research Laboratories, Dong-A Pharmaceutical Co., Ltd.) ;
- Kim, Won-Bae (Research Laboratories, Dong-A Pharmaceutical Co., Ltd.) ;
- Kim, Byong-Moon (Research Laboratories, Dong-A Pharmaceutical Co., Ltd.) ;
- Chang, Mi-Yoon (Department of Life Science, Sogang University) ;
- Yang, Jai-Myung (Department of Life Science, Sogang University)
- 한동표 (서강대학교 생명과학과) ;
- 이택열 (동아제약(주) 연구소) ;
- 김원배 (동아제약(주) 연구소) ;
- 김병문 (동아제약(주) 연구소) ;
- 장미윤 (서강대학교 생명과학과) ;
- 양재명 (서강대학교 생명과학과)
- Published : 1997.12.30
Abstract
Three cDNA fragments located within NS5 region of HCV were synthesized by RT using viral RNA extracted from blood sample of Korean patient as a template. The cDNAs were amplified by PCR, cloned into the T-vector, and the nucleotide sequences were determined. Comparative analysis of the nucleotide and amino acid sequence of NS5 cDNAs showed that it is closely related with HCV type 1b. The cloned NS5 cDNA showed 91-94% homology at the nucleotide sequence level and 96-98% homology at the amino acid sequence level with several strains of the HCV type 1b. The NS5 cDNAs were subcloned into E. coli expression vectors to construct pRSETA5-1, pTHAN5-1, pRSETC5-2, pRSETBB1, pRESTCB1 and pRSETB-H3. Expression of the NS5 proteins was achieved by inducing the promoter with isopropyl-thio-