Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
권호 표지
DOI QR코드

DOI QR Code

Conformation of Single Stranded Poly(dA) and Its Interaction with 4',6-Diamidino-2-phenylindole

  • Published : 1997.05.20
Download PDF
⟨ Previous Next ⟩

Abstract

We studied the interaction of 4',6-diamidino-2-phenylindole (DAPI) with single stranded poly(dA) using optical spectroscopic methods, including absorption, circular dichroism (CD), and fluorescence spectroscopy. The temperature-dependent conformation of poly(dA) was also investigated. The conformation of poly(dA) varied with temperature, which is explained by the stacking-destacking process of the adenine bases, resulting from the sugar conformation. The hypochromicity and red-shift in the absorption spectroscopy, the lack of CD change in the drag absorption region, and the fluorescence behavior, especially a great accessibility of the I2 quencher to the poly(dA)-bound DAPI, suggest that DAPI binds to the outside of poly(dA). The Job plot for the DAPI-poly(dA) mixture demonstrated that a stoichiometry of one DAPI molecule binds to the one phosphate of poly(dA).

Keywords

References

  1. Liebigs Ann. Chem. v.749 Dann, O.;Bergen, G.;Demant, E.;Volz, G.
  2. Nucleic Acids Res. v.6 Kapuscinsk, J.;Yanagi, K.
  3. Nature v.338 Schwarz, D. C.;Koval, M.
  4. Dev. Brain Res. v.50 Brown, R. N.;Hitchcook, P. F.
  5. J. Invest. Dermatol. v.95 Chi, H.;Ishibashi, Y.;Shima, A.;Mihara, I.;Otsuka, F.
  6. Science v.191 Hajduck,S. L.
  7. Anal. Biochem. v.137 Lee, G. M.;Thornthwaite, J. T.;Rasch, E. M.
  8. Nature v.253 Russel, W. C.;Newman, C.;Williamson, D. H.
  9. Acta Histochem. Cytochem. v.23 Takata, K.;Hirano, H.
  10. Biochim. Biophys. Acta v.721 Tijssen, J. P. F.;Beekes, H. W.;van Stevenick, J.
  11. Methods Enzymol. v.56 Williamson, D. H.;Fennell, D. J.
  12. J. Biotech. Histochem. v.1 Bella, J. L.;Gosalvez, J.
  13. Hum. Genet. v.83 Bernheim, A.;Miglierina, R.
  14. Chromosoma v.60 Lin, M. S.;Comings, D. E.;Alfi, O. S.
  15. Biochemistry v.26 Kubista, M.;Akerman, B.;Norden, B.
  16. In the Jerusalem Symposia on the Quantum Chemistry and Biochemistry v.23 Norden, B.;Eriksson, S.;Kim, S. K.;Kubista, M.;Lyng, R.;Akerman, B.;Pullman, B.(Ed.);Jortner, J.(Ed.)
  17. Photochem. Photobiol. v.51 Hard, T.;Fan, P.;Kearns, D. R.
  18. Nucleic Acids Res. v.13 Manzini, G.;Xodo, L.;Barcelona, M. L.;Quadrifoglio, F.
  19. Biochemistry v.29 Wilson, W. D.;Tanious, F. A.;Barton, H. J.;Jone, R. L.;Fox, K.;Wydra, R. L.;Strekowski, L.
  20. J. Am. Chem. Soc. v.111 Wilson, W. D.;Tanious, F. A.;Barton, H. J.;Strekowski, L.;Boykin, D. W.
  21. Biochim. Biophys. Acta v.949 Portugal, J.;Waring, M. J.
  22. J. Biomol. Struct. Dyn. v.7 Larsen, T. A.;Goodsell, D. S.;Cascio, D.;Grzeskowiak, K.;Dickerson, R. E.
  23. J. Am. Chem. Soc. v.115 Kim, S. K.;Eriksson, S.;Kubista, M.;Norden, B.
  24. Anti-Cancer Drug Design v.5 Wilson, W. D.;Tanious, F. A.;Barton, H. J.;Wydra, R. L.;Johns, R. L.;Boykin, D.;Strekowski, L.
  25. J. Biomol. Str. Dyn. v.11 Tanious, F. A.;Spychala, J.;Kumar, A.;Greene, K.;Boykin, D. W.;Wilson, W. D.
  26. Nucleic Acids Res. v.23 Trotta, E.;D'Ambrosio, E.;Ravagnan, G.;Paci, M.
  27. Eur. J. Biochem. v.115 Olsthoorn, C. S. M.;Bostelaar, L. J.;van Boom, J. H.;Altona, C.
  28. Eur. J. Biochem. v.115 Olsthoorn, C. S. M.;Bostelaar, L. J.;Derooij, J. F. M.;van Boom, J. H.;Altona, C.
  29. Inorg. Chem. v.29 Pasternack, R. F.;Brigandi, R. A.;Abrams, M. J.;Wiliams, A. D.;Gibbs, E. J.
  30. Nucleic Acids Res. v.5 Kapuscinski, J.;Skozylas, B.
  31. Principles of Fluorescence Spectroscopy Lakowicz, J. R.
  32. Ann. Chim. v.9 Job, P.
  33. Biophysical Chemsitry v.III Cantor, C. R.;Schimmel, P. R.
  34. Methods Enzymol. v.87 Huang, C. Y.
  35. Biopolymers v.11 Powell, J. T.;Richards, E. G.;Gratzer, W. B.
  36. J. Mol. Biol. v.65 Lowe, M. J.;Schellman, J. A.
  37. Biochemistry v.32 Eriksson, S.;Kim, S. K.;Kubista, M.;Norden, B.
  38. J. Am. Chem. Soc. v.115 Sehlstedt, U.;Kim, S. K.;Norden, B.
  39. Biopolymers v.33 Kim, S. K.;Erkisson, S.;Norden, B.
  40. Biochemsitry v.30 Loontiens, F. G.;McLaughlin, L. W.;Diekmann, S.;Clegg, R. M.
  41. Photochem. Photobiol. v.44 Szabo, A. G.;Krajcarski, D. T.;Cavatorta, P.;Masotti, L.;Barcelonak, M. L.