Journal of Microbiology and Biotechnology
- Volume 7 Issue 6
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- Pages.417-422
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- 1997
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Enhanced Production of Maltotetraose-producing Amylase by Recombinant Bacillus subtilis LKS88 in Fed-batch Cultivation
- KIM, DAE-OK (Department of Food Science & Technology and Research Center for New Bio-Materials in Agriculture, Seoul National University) ;
- KYUNGMOON PARK (Department of Food Science & Technology and Research Center for New Bio-Materials in Agriculture, Seoul National University) ;
- JAE-WOOK SONG (Department of Food Science & Technology and Research Center for New Bio-Materials in Agriculture, Seoul National University) ;
- JIN-HO SEO (Department of Food Science & Technology and Research Center for New Bio-Materials in Agriculture, Seoul National University)
- Published : 1997.12.01
Abstract
Recombinant Bacillus subtilis LKS88[pASA240] containing the amylase gene from Streptomyces albus KSM-35 was exploited in fed-batch cultivation for mass production of maltotetraose-producing amylase. The effects of dissolved oxygen, additional organic nutrients (peptone and yeast extract) and mixed carbon sources (glucose plus soluble starch) on amylase production were examined in fed-batch operations in an effort to determine the optimum conditions for a maximum amylase productivity. Under the optimum conditions, maximum amylase activity was about 4.2 times higher than that obtained in batch cultivations, indicating that mass production of maltotetraose-producing amylase could be accomplished in fed-batch cultivation of the recombinant B. subtilis strain.