Journal of Microbiology and Biotechnology
- 제7권4호
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- Pages.267-271
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- 1997
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Contraction Behavior of Collagen Gel and Fibroblats Activity in Dermal Equivalent Model
- Yang, Eun-Kyung (Department of Chemical Engineering, Dongguk University) ;
- Lee, Doo-Hoon (Department of Chemical Engineering, Dongguk University) ;
- Park, Sue-Nie (Department of Chemical Engineering, Dongguk University) ;
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Choe, Tae-Boo
(Department of Microbial Engineering, Konkuk University) ;
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Park, Jung-Keug
(Department of Chemical Engineering, Dongguk University)
- 발행 : 1997.08.01
초록
We developed a dermal equivalent (DE) which was engineered using human dermal fibroblasts and a matrix of collagen gel. The in vitro construction of the DE was accomplished by casting a porcine collagen type I solution plus concentrated medium with isolated and cultured fibroblasts. These constructs were attached to culture dishes or left floating in culture medium. Contraction of attached gels results in decreased gel thickness without a change in gel diameter, and contraction of floating gels results in decreased gel thickness and diameter. After contraction, there was no increase in cell number in floating gels, but cells in attached gels began to increase after about 4 days of the lag phase in cell growth curve. At this lag phase, addition of fibroblast growth factor (FGF) at a concentration of