Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Production of Lysophospholipid Using Extracellular Phospholipase $A_1$ from Serratia sp. MK1

  • Kim, Jeong-Kyun (Department of Biological Sciences, Korea Advanced Institute of Science and Technology) ;
  • Kim, Myung-Kee (Department of Biological Sciences, Korea Advanced Institute of Science and Technology) ;
  • Chung, Guk-Hoon (Lipid Technology Group, Doosan Serdary Research Laboratories) ;
  • Choi, Choon-Soon (Department of food Technology, Kwangju Health Junior College) ;
  • Rhee, Joon-Shick (Department of Biological Sciences, Korea Advanced Institute of Science and Technology)
  • Published : 1997.08.01
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Abstract

For the efficient production of lysophospholipid the hydrolysis of phospholipid using phospholipase $A_1$ from Serratia sp. MK1 was studied in an aqueous-solvent, a two-phase and an emulsion system. Judged on the basis of productivity and the degree of hydrolysis, the yield of lysophospholipid in a two-phase system was found to be better than that obtained in an emulsion system. Among the 13 organic solvents tested phospholipase $A_1$ showed the most efficient catalytic activity and stability in butyl acetate. When 20% phospholipid was used it was completely hydrolyzed in this two-phase system.

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