Journal of Microbiology and Biotechnology
- 제7권3호
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- Pages.167-173
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- 1997
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Characteristics of ATPases Present in Everted Membrane Vesicles of Helicobacter pylori
- Yun, Soon-Kyu (Department of Biotechnology, Korea University) ;
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Hwang, Se-Young
(Department of Biotechnology, Korea University)
- 발행 : 1997.06.01
초록
Everted membrane vesicles of Helicobacter pylori were prepared and the membrane-resided ATPases were characterized. For comparison, Escherichia coli membrane ATPases and hog gastric mucosal H,K-ATPase were employed. ATPase assay revealed that the composite enzyme pool was relatively low in specific activities, below 1/10 times than that found in E. coli. According to their inhibitory specificities, most of the ATPase pool appeared to belong to the P-type ATPase, sensitive to vanadate but not to azide. The enzyme pool was extraordinarily resistant against treatment by N,N'-dicyclohexylcarbodiimide (DCCD). Certain monovalent cations, e.g.,