Journal of Microbiology and Biotechnology
- Volume 7 Issue 2
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- Pages.138-143
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- 1997
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Expression of de novo Designed High Nutritional Peptide (HEAAE) in Tobacco
- Kim, Jae-Ho (Graduate School of Biotechnology, Korea University) ;
- Lee, Chang-Kook (Utilization Research Lab, National Fisheries Research and Development Agency) ;
- Hong, Bun-Shik (Graduate School of Biotechnology, Korea University)
- Published : 1997.04.01
Abstract
We have designed and constructed a gene encoding novel high essential amino acid encoding protein(HEAAE). The resultant DNA fragment was tested for in vitro and in vivo expression and then cloned into plant expression vector pBI121, under the control of the cauliflower mosaic virus 35S promoter. Agrobacterium tumefaciens, strain LBA4404, was subsequently transformed with this new construct and Nicotiana tabacum var. Xanthi transgenic plants were obtained. DNA analysis by Southern procedure confirmed the presence of the multi-copy number of genes in the transformed plants. Analysis of RNA and protein synthesized in these transgenic plants demonstrated the stable expression of this gene.