Immunosuppressive Characteristics of Oligomycin Derivatives Produced by Streptomyces lydicus MCY-524

  • Lee, Sang-Yong (Department of Microbiology, Kyungpook National University) ;
  • Han, Sang-Bae (Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Kim, Hang-Sub (Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Kim, Young-Ho (College of Pharmacy, Chungnam National University) ;
  • Kim, Hwan-Mook (Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Kim, Chang-Jin (Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Hong, Soon-Duck (Department of Microbiology, Kyungpook National University) ;
  • Lee, Jung-Joon (Korea Research Institute of Bioscience and Biotechnology, KIST)
  • 발행 : 1997.02.01

초록

A strain producing immunosuppressive substances was isolated from a soil in Cheju island. By morphological, cultural, and physiological studies, the strain was identified as Streptomyces lydicus MCY-524. Cultured broth was purified by silica gel, sephadex LH-20 and preparative HPLC and gave two immunosuppressive compounds, MCH-22 and MCH-32. They dramatically suppressed the B cell activation with lipopolysaccharide, T cell activation by mixed lymphocyte response, and primary T-dependent antibody response at a final concentration of 1 ${\mu}g$/ml. They also markedly suppressed the proliferation of lymphocytes induced by lipopolysaccharide, pokeweed mitogen, and concanavaline A at the same concentration. Their suppressive activities, which were comparable to those of cyclosporin A, suggested that they were potent and broad immunotoxic agents on the immune functions of murine lymphocytes.

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