Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Shoot Regeneration from Cambial Tissue Culture of European Larch (Larix decidua)

유럽낙엽송의 형성층조직 배양으로부터 줄기의 재분화

  • SHIN, Dong Ill (Department of Plant Breeding and Genetics, Catholic University of Taegu-Hyosung) ;
  • SUL, Ill-Whan (Institute of Agricultural Science and Technology, Kyungpook National University) ;
  • PARK, Young Goo (Department of Forestry, Kyungpook National University)
  • Published : 1997.11.01
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Abstract

Adventitious shoots were induced from cambial tissue cultures of 3-year-old seedlings using BLG mineral salts medium supplemented with 10 mM glutamine and 30 mM sucrose. The optimum growth regulator level for bud induction was 4,5 $\mu$M BA which produced average 25.5 shoots per cambium segment. Induced buds were elongated on GD medium supplemented with 30 mM sucrose followed by LMG medium supplemented with 30 mM sucrose for further shoot elongation. Elongated shoots were rooted on half-strength GD medium containing $0.54 ;\mu\textrm{M}$ NAA with the frequency of 20%. This system proved the high morphogenic potential of cambial tissue in larch.

3년생 유럽낙엽송의 실생묘로부터 형성층조직을 분리하여 부정아를 유기하였다. BLG배지에 4.5$\mu$M의 BA가 첨가된 배지에서 형성층 절편체 당 약 25개의 부정아가 형성되어 시험된 여러 가지 BA와 NAA농도 중 최고의 발생율을 보였다. 유기된 부정아는 1/2 GD배지와 LMG배지에서 10주 후 약 2.5cm의 신장을 보였다. GD배지에서는 이보다 낮은 약 1,8cm의 신장을 나타내었다. 발근은 NAA가 $0.54\;\mu\textrm{M}$ 함유된 1/2 GD배지에 2주간 배양하였을때 20% 정도 나타났다. 발근된 shoot들은 NAA가 함유되지 않은 1/2 GD 배지에서 뿌리를 신장시킨 후 온실로 옮겨져 순화되었다. 본 실험의 결과 그동안 낙엽송의 조직배양시료로 사용되지 않았던 형성층 조직의 뛰어난 형태형성능을 보여줌으로써 기내 급속대량증식 뿐 아니라 향후 낙엽송의 체세포 변이주선발이나 형질전환 및 그와 관련된 연구분야에 유용하게 사용될 수 있을 것으로 사료된다.

Keywords

References

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