Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Totipotential, Morphological, Biochemical Comparisons between Nonembryogenic Callus and Embryogenic Callus in Water Dropwort(Oenanthe stolonifera DC)

미나리에서 비배발생캘러스와 배발생캘러스간의 분화능력 및 해부학적, 생화학적 특성비교

  • 빈철구 (창원시설화훼시험장 육종재배실) ;
  • 김병동 (서울대학교 농업생명과학대학 원예학과)
  • Published : 1997.05.01
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Abstract

The embryogenic callus (EC), from which somatic embryos could be induced, was compared with nonembryogenic callus(NE) to study the origin and features of totipotent cell in water dropwort (Oenanthe stolonifera DC). To induce and maintain of EC and the NE, meristematic stem and immature floret were inoculated in MS media supplemented with 1 mg/L 2,4-D, and with 2.5 mg/L NAA and 5mg/L BA, respectively, The EC was not induced from the NE even after subculturing in MS medium supplemented with 1 mg/L 2,4-D. Plantlets were not regenerated from the NE in hormone-free medium. In histochemical comparison of the EC with the NE by light microscopy, the EC had smaller cells in size, dense cytoplasm, and more starch granules of cells compared to the NE cells. The cell from the EC, as observed by transmission electron microscopy, had smaller vaculoes, well developed ribosomes, mitochondria, and endoplasmic reticulum, whereas the cells from the NE had larger vacuoles and underdeveloped organelles. In protein pattern from NE, EC and Somatic embryo (SE), as analyzed by SDS polyacrylamide gel electrophoresis, different proteins specific for tissue were observed: 17 and 28 KD for NE, 50, 52, 57, 66, 68 KD for EC and 20 KD for SE. DNA polymorphism was also observed between EC and NE as analyzed by RAPD (randomly amplified polymorphic DNA) method. The origin of totipotent stem cell and the relationship between irreversible genomic change arose in differentiation and the loss of totipotency in plant were discussed.

Keywords

References

  1. Handbook of Plant Cell Culture v.1 Embryogenesis Ammirato PV;DA Evans(eds);WR sharp(eds);PV Ammirato(eds);Y. Yamado(eds)
  2. The Future of the Plant Developmental Engineering v.1 Been CG
  3. Plant Cell Tissue and Organ Culture v.18 Identification and isolation of a unique esterase from the medium of nonembryogenic cell line of cultured carrot cells Chibbar RN;Polowick PL;Newsted WJ;Hyluk JS;Georges F
  4. Korea Soc Hort Sci Abstracts v.14 Selection of organogenic specific gene markers from gladiolus callus by using amplified fragment length polymorphism (AFLP) Choi JD;Suh SK;Kim KW
  5. Proc Natl Acad Sci USA v.84 The coloning of genes developmentally regulated during plant embryogenesis Choi,JH;Liu L;Borkird C;Sung ZR
  6. Plant Genetic Transformation and Gene Expression The isolation of plant nucleic acids Draper J;Scott S;J. Draper(eds);S. Scot(eds);P.Armitage(eds);R.Walden(eds)
  7. Cell Culuturd and Somatic Cell Genetics of Plant v.3 Attainment and Retintion of Morphogenetic capacity in vitro Halperin W;IK Vasil(ed)
  8. J Ultrastruct Res v.18 Ultrastructural changer during growth and embryogenesis in carrot cell cultures Harperin W;Jensen WA
  9. Theor Appl Genet v.62 Loss of nuclear DNA in leaves of rye Hesemann CU;Schr der G
  10. Korea J Plant Tissue Culture v.1 Protein analysis and structural aspects somatic embryogenisis in Oenanthe javanica Kim SY;Cho RY;Son WY
  11. Biology(Ed 5 ) Kimball JW
  12. Nature v.326 Differentiated parental DNA strand confer developmental asymmetry on daughter cells in fission yeast Klar AJS
  13. Korea J Plant Tissue Culture v.22 Production and developmental pattern of embryogeneic callus in Oenanthe javanica (BL) DC Koh GC;Ahn CS
  14. KoreaJ Plant Tissue Culture v.22 Anatomical obeservation of somatic embryogenesis in Oenanthe javanica (BL) DC Koh GC;Ahn CS
  15. Bot Rev v.31 Plant Cell and Tissue Cultlure: Role of Harberlandt Krikorian AD;Berguam DL
  16. J Korean Soc Hort Sci v.34 Development of mass production system by somatic embryos in Oenanthe stolonifera DC Lee GW
  17. Plant Cell Reports v.8 Embryogenic and non-embryogeneic cell lines of Daucus carota cloned from meristematic cell clusters relation with cell ploidty determinated by flow cytonety Loutos-Thevnot P;Jouarmmeau JP;Brown S;Peitard V;Guerm V
  18. Physiol Planrarum v.15 A revised medium for rapid growth and bioassay with tobacco tissue culture Murashige T;Skoog F
  19. Z Pflanzenphysiol v.105 Green spots are predictors of high callus growth rates and shoot formation in normal and in salt stressed tissue culture of oat Nabors MW;Kroskey CS;McHugh DM
  20. Protoplasm v.118 Heterochromatin elimination in the Orchid dendrobiumi Nagl W
  21. Ann Bot v.38 The decline of embryogenic potential as callus suspension cultures of carrot (Daucus acrota L.)are serially subcultured Smith SM;Street HE
  22. Am J Bot v.73 Meristematic precusors of vascular parenchim differentiate from $G_2$ phase after replicating DNA discontinuously Vant Hof J;Bjerknes CA;Lamm SS
  23. Plant Physiol v.52 Flower formation in excised tobacco stem segments.III. DNA content in stem tissue of vegetative and flowering tobacco plant Wardell WL;Skoog F