Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Growth and Tropane Alkaloid Production of Hairy Roots of Datura stramonium var. tatula Torr. Transformed by Agrobacterium spp.

Agrobacterium spp.에 의하여 형질전환된 독말풀(Datura stramonium var. tatula Torr.) 모상근의 성장과 tropane alkaloid의 생성

  • 양덕조 (충북대학교 자연과학대학 생명과학부) ;
  • 강현미 (충북대학교 자연과학대학 생명과학부) ;
  • 이강섭 (충북대학교 자연과학대학 생명과학부) ;
  • 김용해 (충북대학교 자연과학대학 생명과학부) ;
  • 양덕춘 (한국인삼연초연구소)
  • Published : 1997.05.01
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Abstract

In order to elucidate the optimum medium on the growth and tropane alkaloid production of hairy root, hairy root were induced by inoculating leaf and stem of Datura stramonium var. tatula Torr. with Agrobacterium spp. Both Agrobacterium tumefaciens $\textrm{A}_{4}$ T and A, rhizogenes ATCC 15834 among tested strains were effective on hairy root formation. Among 23 clones selected in SH (Schenk and Hildebrandt, 1972) liquid medium, DTLA9 clone was shown fast growth of hairy root and DTLE6 clone was shown high level production of tropane alkaloids. When both DTLA9 and DTLE6 clones were cultured in the GD (Gresshoff and Doy, 1972) medium, alkaloid production was higher than in 8 tested media. It was elucidated that optimum medium for root proliferation and for tropane alkaloid production is SH, GD medium, respectively.

모상근의 성장을 위한 최적배지 및 tropane alkaloids함량을 위한 최적배지를 구명하기 위하여, 독말풀(Datura stramonium var. tatula Torr.)의 잎과 줄기에 Agrobacterium spp.spp.를 접종하였다. 접종된 균주 중 모상근의 유도에 효과적인 균주는 Agrobacterium tumefaciens $\textrm{A}_{4}$ T와 A rhizogenes ATCC 15834이었다. SH (Schenk and Hildebrandt, 1972) 액체배지에서 선발된 23개의 clone중 성장이 양호한 clone은 DTLA9 이었으며, tropane alkaloids의 함량이 양호한 clone은 DTLE6 이었다. 이들 두 종류의 세포주를 9종류의 서로 다른 액체배지에 각각 배양하여 성장 및 tropane alkaloids의 함량을 조사한 결과, 모상근의 성장은 두 세포주 모두 SH와 MS배지(SH > MS배지순)에서 다른 7개의 배지에서 보다 양호하였다. 한편, tropane alkaloids의 함량은 두 세포주 공히 GD와 B5 배지(GD > B5배지순)에서 다른 7개의 배지에서 보다 높게 나타났다. 따라서 SH배지에서 모상근을 성장시킨 후, GD배지에 옮겨 2단계 연속배양하면 tropane alkaloids의 함량을 높일 수 있을 것으로 확인되었다.

Keywords

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