Biomedical Science Letters (대한의생명과학회지)
- Volume 3 Issue 2
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- Pages.169-175
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- 1997
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- 1738-3226(pISSN)
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- 2288-7415(eISSN)
Changes of Glucose-6-Phosphate Dehydrogenase on Alloxan-induced Diabetic Rat
Alloxan으로 유발된 당뇨쥐의 간조직 Glucose-6-Phosphate Dehydrogenase 효소의 변화
Abstract
The hypoglycemic and metabolic effects of Commelina communis L. extract were investigated in alloxan-induced diabetic rats. The increased blood glucose level in the diabetic rats was sinificantly lowered with the treatments of the plant protein extract. Administration of the plant extract ellicited the significant increase of glucose-6-phosphate dehydrogenase (G6PD) activity in liver of alloxan-induced rats. Three isozyme patterns(band I, II & III : in order decreasing mobility) of G6PD were found when normal rat liver extract were subjected to electrophoresis on native polyacrylamide gel. On the other hand, G6PD band patterns of alloxan-induced rat liver extract were found band II isozyme missing. By treatment of plant extract in alloxan-induced rats has been showed pattern the recovery of missing band patterns. This indicates that changes of the G6PD isozyme might be related to the cellular process of diabetes.
닭의장풀 추출액을 쥐의 체중 1 kg 당 40 mg의 alloxan을 미정맥 주사한 당뇨쥐를 실험군으로 하여 혈당강하효과를 보았다. 정상대조군에게는 0.9% saline용액을 투여하고 당뇨쥐에게는 쥐의 체중 1 kg당 100 mg의 식물단백추출액을 경구투여하여 683.6