Biomedical Science Letters (대한의생명과학회지)
- Volume 3 Issue 2
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- Pages.107-114
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- 1997
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- 1738-3226(pISSN)
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- 2288-7415(eISSN)
Development of Enzyme Immuno Assay for Analysis of Free Prostate Specific Antigen in Serum
혈청 유리형 전립선항원 (free PSA) 측정을 위한 효소면역측정법의 개발
- Kyung-Ok Lee (Department of Immunology, Seoul Medical Science Institute Seoul Clinical Laboratories (SCL)) ;
- Kyung-In Kim (Department of Immunology, Seoul Medical Science Institute Seoul Clinical Laboratories (SCL)) ;
- Kyu-Pum Lee (Department of Immunology, Seoul Medical Science Institute Seoul Clinical Laboratories (SCL))
- Published : 1997.12.01
Abstract
Recent reports indicate that the clinical usefulness of prostate specific antigen (PSA), particulary in the differentiation of benign prostate hyperplasia from prostate cancer, can be improved by measuring the amount of free PSA in serum. Measuring free PSA is especially useful in attempts to improve diagnositc performance of PSA in the diagnostic gray zone of total PSA. The objective of this study was to develop free PSA assay kit using sandwich microplate enzyme immunoassay format. We chose a test format with polyclonal anti-PSA antibodies coated on the wells and monoclonal anti-free PSA antibodies for quantification to gain higher test sensitivity. We adpoted 10 uL of specimen and 2 hours of first incubation time with detecting antibody for free PSA EIA format using microplate. The within-day and between-day precision (%CV) in the high and low concentration ranges were below 4%. The correlation coefficient between in-house free PSA assay and commercial assay kit was r=0.9965 (slope=0.0984, y intercept=0.0173, N=27). No hook effect was found by 40 ng/mL and correlation coefficient (r) value of the fitted linear regression was over 0.995. The recovery tests were in the range of 98.9∼104.1% for free PSA. In conclusion, in-house free PSA enzyme immune assay is cost effective, simple and rapid and could be useful for the prognosis after theraphy as well as for the differential diagnosis between prostate cancer and benign prostate hyperplasia.
전립선특이 항원 (PSA: Prostate Specific Antigen)은 전립선 세포에서 분비되는 단백질로서 장기 특이성이 높아 전립선암의 조기진단 및 치료의 예후를 판단하기 위해 임상에서 널리 사용되고 있는 혈청 종양표지검사의 대상항원이다. 그러나 혈청 PSA치는 전립선암 뿐 아니라 양성 전립선질환인 전립선비대증 (BPH), 전립선 경색, 전립선 염증 등에서도 상승될 수 있다. 혈청 내의 PSA는 여러 가지 분자형태로 이루어져 있으며 대표적으로