Korean journal of food and cookery science (한국식품조리과학회지)
- Volume 13 Issue 4
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- Pages.422-426
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- 1997
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- 2287-1780(pISSN)
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- 2287-1772(eISSN)
Effect of Processing on the Content of Sulforaphane of Broccoli
브로콜리의 조리가공에 따른 sulforaphane 함량
Abstract
Fresh broccoli is known to have the highest content of sulforaphane (S-methylsulfinylbutyl isothiocyanate) among all vegetables. Since isothiocyanates are formed from myrosinase-catalyzed hydrolysis of glucosinolates during tissue destruction of broccoli, the formation of sulforaphane in the extract of broccoli was examined under various processing conditions. The amount of sulforaphane in processed broccoli was measured using GC/MS analysis. Among fresh, dried, and boiled broccoli fresh broccoli exhibited the highest content of sulforaphane. Sulforaphane was maximally produced from the homogenate in 0.1 M phosphate buffer containing 1 mM Vitamin C stored at room temperature for 1 hr. In boiled broccoli, the amount of sulforaphane decreased as the boiling time increased, and reached to 10% of control after 30 min boiling. The amount of sulforaphane was decreased remarkably in dried broccoli in which freeze-dried and heat-dried broccoli had about 50% and 30% of fresh ones, respectively.
브로콜리의 조리가공 방법에 따른 sulforaphane(4-methylsulfinylbutyl isothiocyanate) 생성량을 GC/MSD로 분석하여 chemoprotection에 가장 효과적인 조리방법을 모색하고자 하였다. Sulforaphane량이 가장 많은 브로콜리의 조리 가공방법은 생즙(juice)이었으며, 브로콜리 생즙 제조시 sulforaphane최대 함유조건은 Vitamin C, 1mM이 첨가된 pH 7 buffer에서 마쇄후 실온에서 1시간 방치하였을 때이었다. 가열시(10