Micronucleus Test of DW-166HC, a Novel Radiopharmaceutic Anticancer Agent

신규 방사성 항암제 DW-166HC의 소핵시험

  • Moon, Eun-Yi (Research Laboratories of Dong-Wha Pharm. Ind. Co. Ltd.) ;
  • Lee, Jin (Research Laboratories of Dong-Wha Pharm. Ind. Co. Ltd.) ;
  • Lee, Won-Yong (Research Laboratories of Dong-Wha Pharm. Ind. Co. Ltd.) ;
  • Choi, Chung-Ha (Research Laboratories of Dong-Wha Pharm. Ind. Co. Ltd.) ;
  • Lee, Dog-Keun (Research Laboratories of Dong-Wha Pharm. Ind. Co. Ltd.) ;
  • Ryu, Jei-Man (Research Laboratories of Dong-Wha Pharm. Ind. Co. Ltd.) ;
  • Chung, Yong-Ho (Research Laboratories of Dong-Wha Pharm. Ind. Co. Ltd.) ;
  • Yoon, Sung-June (Research Laboratories of Dong-Wha Pharm. Ind. Co. Ltd.) ;
  • ark, Kyung-Bae (Korea Atomic Energy Research Institute)
  • 문은이 (동화약품공업(주) 중앙연구소) ;
  • 이진 (동화약품공업(주) 중앙연구소) ;
  • 이원용 (동화약품공업(주) 중앙연구소) ;
  • 최청하 (동화약품공업(주) 중앙연구소) ;
  • 이덕근 (동화약품공업(주) 중앙연구소) ;
  • 유제만 (동화약품공업(주) 중앙연구소) ;
  • 정용호 (동화약품공업(주) 중앙연구소) ;
  • 윤성준 (동화약품공업(주) 중앙연구소) ;
  • 박경배 (한국원자력연구소)
  • Published : 1997.09.01

Abstract

DW-166HC ($^{166}$ Holmium ($^{166}$ Ho)-Chitosan complex) is a new radiopharmaceutic anticancer agent with a broad anti-tumoriginec spectrum, especially against human fepatic cancer. DW-166HC was evaluated for the appearance of micronucleus in polychromatic erythrocytes (PCEs) of mouse bone marrow cells after subcutaneous and intravenous single administration. Bone marrow cells were prepared at 24 hr and 48 hr after DW-166HC-I ($^{165}$ Ho-Chitosan complex cold compound) administration and at 24 hr, 72 hr and 2 weeks after DW-166HC ($^{166}$ Ho-Chitosan complex : hot compound) administration. The results showed there was no statistically significant increase of the numbers of PCEs with micronucleus in all DW-166HC-I administered groups compared with a negative control group but there was statistically significant increase of the numbers of PCEs with micronucleus at 24 hr and 72 hr in all DW-166HC administered groups, which was recovered after 2 weeks from the drug administration. The results also showed the ratio of normochromatic erythrocytes (NCEs) to PCEs of all DW-166HC-I administered groups was not significantly different from that of a negative control group but there was significant difference this ratio at 24hr and 72 hr in all DW-166HC administered groups compared with that of negative group, which was also recovered after two weeks from the drug administration. These results suggested that DW-166HC-I may not cause any chromosomal damage but DW-166HC has in vivo mutagenic potential because of its radioactivity.

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