Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
권호 표지

Study on the Hemolysin from Marine V. vulnificus

해양 V. vulnificus의 Hemolysin에 관한 연구

  • Published : 1997.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

A halophilic V. vulnificus is an estuarine microorganism that has been associated with fatal wound Infection and life-threatening septicemia. Hemolysin is defined as toxic substance produced by various species of bacteria Including V. vulnificus. Hemolysin from marine V. vulnificus was purified and the effect of pH, temperature. metal ion on the activity of hemolysin, and thermostability of hemolysin were tested in this study. Hemolysin iysed the sheep red blood cell and the optimum pH was 8.0, the optimum temperature was 4$0^{\circ}C$, and $K^+$ increased but $Mn^{2+}$ decreased the hemolyic activity of hemolysin, but hemolysin was unstable to heat.

호염기성 V. vulnificus는 연안에 존재하는 미생물로서 치명적인 상처 감염과 생명을 위협하는 패혈증과 관련이 있다. Hemolysin은 V. vulnificus를 포함하는 여러 가지 세균종에 의해 생성되는 독성 물질이다. 본 연구에서는 해양 V. vulnificus에서 hemolysin을 정제한 후 hemolysin의 활성에 미치는 pH, 온도 및 금속 이온의 영향을 조사하였다. Hemolysin은 sheep red blood cell을 용혈시켰으며 hemolysin의 최적 pH는 8.0, 최적 온도는 4$0^{\circ}C$이었으며 $K^+$ 이온은 homolysin의 활성을 증가시켰으나 $Mn^{2+}$는 감소시켰다. 그러나 hemolysin은 열에 불안정하였다.

Keywords

References

  1. Media Circle v.29 Azuma, I.;Kitaura, T.;Harada, K.;Dohke, S.;Yabuuchi, E.
  2. Infection and Im-munity v.52 Bhakdi, S.;Mackman, N.;Nicaud, J.;Holland, I.
  3. Ann. Rev. Microbiol. v.34 Disease of humans(other than choera)caused by vibrios Blake, P. A.;Weaver, R. E.;Hollis, D. G.
  4. J. Cell. Biology v.91 Fussle, R.;Bhakdi, S.;Sziegolett, A.;Jen-sen, J.;Kranz, T.;Wellensiek, H.
  5. Infection and Im-munity v.48 Purification and charaterization of an ex-tracellular cytolysin produced by Vi-brio vulnificus Gray L.;Kreger, A.
  6. J. Clin. Microbiol. v.14 Johnson, D. E.;Calia, F. M.
  7. Infection and Im-munity v.33 De-tection of extracellular toxins pro-duced by Vibrio vulnificus Kreger A.;Lockwood, D.
  8. FEMS Microbiology Letters v.30 Non-thio ac-tivated property of a cholesterol-bind-ing hemolysin produced by Vibrio vul-nificus Miyoshi, S.;Yamanaka, H.;Miyoshi, N.;Shinoda, S.
  9. J. of Medicine v.282 Roland, F. P.
  10. Microbiology and Immunology v.29 Some pro-perties of Vibrio vulnificus hemolysin Shinoda, S.;Miyoshi, S.;Yamanaka, H.;Miyoshi, N.
  11. Infection and Im-munity v.50 Wright, A. C.;Morris, J. G.;Maneval, D. R.;Richardson, K.;Kaper, J. B.