Anticlastogenicity of $\beta$-Carotene and Galangin using in vivo Supravital Staining Micronucleus Test

In vivo Supravital Staining Micronucleus Test에 의한 $\beta$-Carotene과 Galangin의 소핵생성억제효과

  • 허문영 (한국과학기술원 도핑콘트롤센타) ;
  • 김정한 (강원대학교 약학과) ;
  • 류재천 (한국과학기술원 도핑콘트롤센타)
  • Published : 1997.09.01

Abstract

The micronucleus test using peripheral blood reticulocytes (RETs) was evaluated in ICR mice treated with N-methyI-N-nitrosourea (MNU) and benzo(a)pyrene [B(a)P] as model clastogens. The frequency of micronucleated reticulocytes (MNRETs) in both positive compounds was similar to other results which were reported previously. On the other hand, an anticlastogenic effect of the natural antioxidant, $\beta$-carotene and one of taroholds, galangin as model anticlastogens were investigated using simultaneous treatment. Mice were treated with a model clastogen alone, or with a model clastogen and a model anficlastogen simultaneously. Both $\beta$-carotene and galangin showed anticlastogenic effects against MNU- or B(a)P-induced micronuclei in mice. However, galangin has stronger activity than $\beta$-carotene. Results from our experiment suggest that the in vivo supravital staining micronucleus test using peripheral blood is useful in the evaluation of clastogenic and anticlastogenic effects.

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