Environmental Analysis Health and Toxicology
- Volume 12 Issue 3_4
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- Pages.15-22
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- 1997
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- 2671-9525(eISSN)
The Characteristics of Fish Metallothionein and Its Application to the Biomonitoring for the Evaluation of Water Pollution
어류 Metallothionein의 툭성 및 수질오염 평가를 위한 생물모니터링에의 응용
Abstract
This experiment was performed to examine the immuno-reactive characteristics of fish metal-binding protein, metallothionein (MT), and gain the practical understandings for the proposed use of fish MT as a biomarker. Liver MT induced by Cd in the silver carp was seperated and purified by gel filtration chromatography and ion exchange chromatography. The immuno-reactivity of fish MT was examined with 3 rabbit antisera. Fish MT showed little reactivity with rabbit anti-rat MT antiserum and a weak reactivity with anti-MT peptide antiserum while showed a strong reactivity with rabbit anti-fish MT antiserum. The time-course change of liver MT in the silver carp, after waterborne exposure to 1 ppm of Cd, was checked by Cd-hem method and established competitive ELISA. In both cases, the induction of liver MT showed a good increasing relationship with the exposure days. The results indicate that the fish MT can be developed as a useful biomonitoring means in the toxicological study and for the evaluation of water pollution.
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