인공 산성비가 소나무속의 동위효소 양상에 미치는 영향

Isozyme Variants in Genus Pinus by Simulated Acidic Rain

  • 발행 : 1997.12.01

초록

산성비가 소나무, 리기다 소나무, 잣나무 잎의 동위효소 양상에 미치는 영향을 알아보기 위하여 pH 5.6을 대조구로 pH 3.5의 인공 산성비를 9개월간 처리하여 $\alpha$-Est, POD, GDH의 변화를 조사하였다. pH 3.5의 인공산성비 처리 9개월째에 $\alpha$-Est에 있어서 소나무는 동위효소의 활성에 별 변화가 없었으나, 리기다 소나무는 2개의 새로운 동위효소가 활성화되었으며, 잣나무는 2개의 새로운 동위효소가 활성화되고 5개의 동위효소는 활성이 보여지지 않았다. POD에 있어서도 소나무는 변화를 보이지 않았으나 리기다 소나무는 8개의 동위효소가 새로이 활성화되었으며, 잣나무는 4개의 동위효소의 활성이 보여지지 않았다. GDH 역시 소나무에서는 변화가 없었으나, 리기다 소나무는 2개의 새로운 동위효소가 활성화 되었고, 잣나무는 변화를 보이지 않았다.

The effect of acid rain on plant isozyme response was studied with the treatment of simulated acid rain to Pinus densiflora, P. nigida and P. koraiensis for 9 months. The isozyme pattern of $\alpha$-Esterase($\alpha$-Est), Peroxidase(POD) and Glutamate dehydrogenase(GDH) were observed in the control (pH 5.6) and simulated acid rain (pH 3.5) treatment. No changes of isozyme pattern in $\alpha$-Est was observed in P. densiflora after 9 month treatment of simulated acid rain, but, two new isozymes were activated in P. nigida in the same treatment. In P. koraiensis, two new isozyme were activated but five isozymes were not activated. P. densiflora did not show any difference in POD and GDH after the treatment of simulated acid rain. P. nigida showed activation of eight and two isozymes in POD and GDH, respectively. P. koraiensis showed inactivation of 4 isozymes in POD but showed no changes In GDH.

키워드

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