Isozyme Variants in Genus Pinus by Simulated Acidic Rain

인공 산성비가 소나무속의 동위효소 양상에 미치는 영향

  • Published : 1997.12.01

Abstract

The effect of acid rain on plant isozyme response was studied with the treatment of simulated acid rain to Pinus densiflora, P. nigida and P. koraiensis for 9 months. The isozyme pattern of $\alpha$-Esterase($\alpha$-Est), Peroxidase(POD) and Glutamate dehydrogenase(GDH) were observed in the control (pH 5.6) and simulated acid rain (pH 3.5) treatment. No changes of isozyme pattern in $\alpha$-Est was observed in P. densiflora after 9 month treatment of simulated acid rain, but, two new isozymes were activated in P. nigida in the same treatment. In P. koraiensis, two new isozyme were activated but five isozymes were not activated. P. densiflora did not show any difference in POD and GDH after the treatment of simulated acid rain. P. nigida showed activation of eight and two isozymes in POD and GDH, respectively. P. koraiensis showed inactivation of 4 isozymes in POD but showed no changes In GDH.

산성비가 소나무, 리기다 소나무, 잣나무 잎의 동위효소 양상에 미치는 영향을 알아보기 위하여 pH 5.6을 대조구로 pH 3.5의 인공 산성비를 9개월간 처리하여 $\alpha$-Est, POD, GDH의 변화를 조사하였다. pH 3.5의 인공산성비 처리 9개월째에 $\alpha$-Est에 있어서 소나무는 동위효소의 활성에 별 변화가 없었으나, 리기다 소나무는 2개의 새로운 동위효소가 활성화되었으며, 잣나무는 2개의 새로운 동위효소가 활성화되고 5개의 동위효소는 활성이 보여지지 않았다. POD에 있어서도 소나무는 변화를 보이지 않았으나 리기다 소나무는 8개의 동위효소가 새로이 활성화되었으며, 잣나무는 4개의 동위효소의 활성이 보여지지 않았다. GDH 역시 소나무에서는 변화가 없었으나, 리기다 소나무는 2개의 새로운 동위효소가 활성화 되었고, 잣나무는 변화를 보이지 않았다.

Keywords

References

  1. Water Air and Soil Pollution v.33 Response of two western canadian conifers to simulated acidic precipitation Abouguendia, Z. M.;L. A. Baschak
  2. Hortscience v.21 Isozyme analysis procedures for stone fruits, almond, grape, walnut, pistachio and hig. Arulsekar, S.;E. Parfitt
  3. Crop Sci. v.19 Isozyme patterns in tobacco plant parts and their derived calli Bassiri, A.;P.S. Carlson
  4. Euphytica v.26 Identification of broad bean cultivars based on isozyme paters Bassiri, A.;I. Rouhent
  5. Cytometry v.9 Flow cytometric measurement of pollutant stresses on algal cells Berglund, D.L.;S. Eversmann
  6. Crop. Sci. v.11 Electrophoretic patterns among alfalfa seed proteins from selected varieties, expermental stocks, and species accessions Bingham, E. T.;K. J. Yeh
  7. Heredity v.73 Allozyme and chloroplast DNA variation in Italian and Greek populations of Pinus leucodermis Boscherini, G.;M. Morgante;P. Rossi;G. G. Vendramin
  8. Annu. Rev. Biochem. v.59 Defence-related proteins in higher plants. Bowles, D. J.
  9. SNSF Project As. Norway Res. Rep. FR. v.6 no.76 Impact of acid precipitation on forest and freshwater ecosystems in Norway Braekke, F. H.
  10. J. Amer. Soc. Hort. Sci. v.106 Electrophoretic characterization of strawberry cultivars Bringhurst, R. S.;S. Arulsekar, Jr.;Hancock;V. Voth
  11. J. Korean For. Soc. v.84 Change in needle chlorophyll fluorescence of pinus densilflora and pinus thunbergii trated with artifical acid rain. Choi, Y. B.
  12. 동국대 박사 학위 논문 인공산성우가 소나무 유묘 및 개나리 유목묘의 생장, 식물체내 함유성 성분 및 토양의 화학적 성질에 미치는 영향 정용문
  13. Plant Physiol. v.107 A beta-glucosidase from lodgepole pine xylem specific for the lignin precursor coniferin Dharmawardhana D. P.;B. E. Ellis;J. E. Carlson
  14. Bot. Mag. tokyo v.85 Application of the nadi reaction to rice-peroxidase isozyme stain Endo, Y.
  15. Environmental Pollut. v.22 Peroxidase activity in plant leaves exposed to gaseous HCL or ozone Endress, A. G.;S. J. Suarez;O. C. Taylor
  16. Am. J. Bot. v.63 Effects of simulated acid rain on Phaseolus vulgalis L. (Fabaceae) Ferenbaugh, R. W.
  17. Biochem, Genet. v.8 Genetic relationships between the multiple alchol dehydrogenasas of maize. Freeling, M.;D. Schwartz
  18. Environ. Res. v.69 Differential responses of certain linchen species to sulfur-containing solutions under acidic conditions as expressed by the production of stress-ethylene Garty J.;M. Kauppi;A. Kauppi
  19. Short-long conifer needle syndrome Gordon, C. C.
  20. New Phytol. v.99 Direct folia effects of simulated acid rain. Ⅱ. Leaf surface characteristics. Haines, B. L.;J. A. Jernstedt;H. S. Newfeld
  21. Forest Service Gen. Tech. Reo. PSW-43 Acid rain: threshold of leaf damage in eight species from a forest succession. In Effects affair pollution on mediterranean and temperate forest ecosystem Haines, B. L.;M. Stefani;F. Hendrix;P. R. Miller(ed.)
  22. Planta v.111 Organ specific multiple forms of glutamic dehydrogenase in Medicago sativa Hartmann, T.;M. Nagel;H. I. Ilert
  23. Am. J. Bot. v.67 Response of bush bean exposed to acid mist Hindawi, I. J.;J. A. Rea;W. L. Griffis
  24. Mol. Gen. Genet. v.255 Molecular cloning and characterization of cDNA for anionic and neutral peroxidases from suspensioncultured-cells of sweet potato and their differential expression in response to stress Huh, G. H.;S. J. Lee;Y. S. Bae;J. R. Liu;S. S. Kwak
  25. Crop Science v.10 Genetics of isozyme variants in barley Ⅰ. Esterase Kahler A. L.;R. W. Allard
  26. Biochimic. v.70 Purification and properties of peroxidase from Pinus pinaster needles Karmali, A.;L. R. Santos
  27. J. Korean For. Soc. v.80 Effects of artifical acid rain on seed germination and seedling growth of several conifers. Ⅰ. Kim, G. T.
  28. Korean Biochem. J. v.27 Selection of plant cell lines for high yield of peroxidase Kim S. K.;S. S. Kwak;K. H. Jung;S. R. Min;I. H. Park;J. R. Liu
  29. J. Plant Biol. v.37 Growth response of Ginkgo biloba and Pinus thunbergii Exposed on simulated acid rain Kim M. R.;W. Y. Soh
  30. J. Plant biol. v.38 Effect of simulated acid rain on faliar structural of changes of Ginkgo biloba and Pinus thunbergii Kim M. R.;W. Y. Soh
  31. Korean J. Plant Tissue Culture v.21 Improvement of peroxidase productivity by optimization of medium composition and cell inoculum size in suspension culture of sweet potato(Impomoea batatas) Kwak, S. S.;D. K. Kim;K. H. Jung;S. H. Yoo;I. H. Park;J. R. Liu
  32. Plant Mol. Biol. v.34 Expression of a wheet ADP-glucose phyrophosphorylase gene during development of normal and water-stress affected anthers Lalonde, S.;D. Morse;H. S. Saini
  33. Forest Sci. v.25 The effect of simulated acid rain on seedling emergence and growth of eleven woody species Lee, J. J.;D. E. Weber
  34. 서울대 농학연구 v.9 인공 산성우가 소나무와 잣나무 유묘의 엽록소 함량에 미치는 영향 이돈구;김갑태;신준환;주광앙
  35. Phytochem. v.11 Evidence for conformational changes in grape catechol and oxidase Lerner, H. R.;A. M. Mayer;E. Harel
  36. Biochem. Genet. v.21 Isozyme variation and hetero zygosity in Pinus halepensis L. Loukas, M.;Y. Vergini;C. B. Krimbas
  37. Planta v.169 De-novo synthesis and release of peroxidase in cell suspension cultures of Nicotiana tabacum L. Mader M.;C. Walter
  38. Histochem. v.56 Studies on the physiology of pollen and pollentube growth. Mehan, M.;C. P. Malik
  39. Arch. Environ. Contam. Yoxicol. v.15 Increase in Pinus strobus meedle transectional areas in response to acid misting Maurice, C. G.;R. E. Crang
  40. Plant and Soil v.74 Effects of similated acid rain on germination and early growth of Doulgas-fir and Ponderosa pine. McColl, J. G.;R. Johnson.
  41. Biochem. Genet. v.20 Identification of aneuploids in Nicotiana tabacum by isozyme banding patterns Moore, G. A.;G. B. Collins
  42. Japan J. Genet. v.53 Identification of monosomic and nullisomic oats using leaf peroxidase isozymes. Morikawa, T.
  43. J. Biol. Chem. v.250 High resolution two-dimensional electrophoresis of proteins O'Farrell, P.H.
  44. New Phytol. v.108 Effects of simulated acid rain on leaf wettability, rain retention and uptake of some inorganic ions Percy, K. E.;E. A. Baker
  45. Proc. Natl. Acad. Sci. U. S. A. v.92 Polymorphic simple sequence repeat regions in chloroplast genomes: applications to the population genetics of pines Powell W.;M. Morgante;R. McDevitt;G. G. Vendramin;J. A. Rafalski
  46. Genetics v.68 The developmental genetics of the calcaroides gene in barley Ⅱ. Peroxidase activity in mutant and normal plants at progressive stages of development Price, H. J.;G. L. Stebbins
  47. Environ. Exp. Bot. v.23 Response of tree seedlings to acid precipitation Ⅱ. Effects of simulated acidified canopy throughfall on sugar maple seedling growth Raynall D.J.;J.R. Roman;W.M.Eichenlaub
  48. Genetika v.30 Segregation disturbance in chronically irradiated populations of Pinus sylvestris L. growing in the area of the Chernobyl nuclear power plant accident. Rubanovich, A. V.;V. A. Kal'chenko
  49. Bull. Environ. Contam. Toxicol. v.48 Performance of Vicia faba plants in relation to simulated acid rain and / or endsulfan treatment Singh N.;M. Yunus;K. J. Ahmad
  50. Maize Genetics Cooperation News Letter v.55 Compilation of isozyme genotypes for 342 inbred lines. Stuber, C. W.;M. M. Goodman
  51. Plant Cell Physiol. v.31 Characterization of polypeptides that accumulate in cultured Nicotiana tabaccum cells Takeda, S.;F. Sato;K. Ida;Y. Yamada
  52. Water Air and Soil Pollut. v.7 Short-term effects of a simulated acid rain upon the growth and nutrient relations of Pinus strobus L. Wood, T.;F. H. Bormann
  53. J. Chem. Tech. Biotechnol. v.38 Production of horse radish peroxidase by plant cell culture. Yamada Y.;S. Kobayashi;K. Watanabe;U. Hayashi
  54. Genetics v.142 A comparison of isozyme and quantitative genetic variation in Pinus contortas sp. latifolia by FST Yang, R. C.;F. C. Yeh;A. D. Yanchuk
  55. J. Oriental Bot. Res. v.1 Estimation of genetic relationships and Characterization among Codonopsis lanceolata Benth. et Hook. fil. geographical variety by the electrophoretic patterns of isozymes. Yoon, E. S.;S. R. Lee;Y. S. Lee
  56. Theor. Appl. Genet. v.59 Low temperature sffect on selective fertilization by pollen mixutres of wild and cultivated tomato species Zamir, D.;S. D. Tanksley;R. A. Jones