Journal of the Korean Magnetic Resonance Society (한국자기공명학회논문지)
- Volume 1 Issue 1
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- Pages.21-29
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- 1997
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- 1226-6531(pISSN)
A Debate on the Use of Presaturation Method in NMR for Structure Determination of Polypeptides in $H_2O$
- Lee, Chulhyun (Magnetic Resonance Group, Korea Basic Science Institute) ;
- Yi, Gwan-Su (Magnetic Resonance Group, Korea Basic Science Institute) ;
-
Kim, Eun-Hee
(Magnetic Resonance Group, Korea Basic Science Institute)
;
- Lee, Jo-Woong (Department of Chemistry, Seoul National University) ;
- Chagjoon Cheong (Magnetic Resonance Group, Korea Basic Science Institute)
- Published : 1997.06.01
Abstract
Three typical solvent suppression methods employed for measuring the NOE data that are used for structure determination of polypeptides by modeling were discussed and compared with one another. In the experiments with several peptides composed of 10 to 65 amino acids the presaturation method was found to give severely distorted signal intensities of exchangeable protons, thus making the results based on this method much less reliable.
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