랫드 간장에서 Creatine 섭식에 의한 metabolites의 변화

Effects of Dietary Creatine on Tissue Metabolite Contents and Uptakes in Rat Liver

  • 정규식 (한국과학기술연구원 생명공학연구소) ;
  • 박상준 (한국과학기술연구원 생명공학연구소) ;
  • 민병길 (한국과학기술연구원 생명공학연구소) ;
  • 류시윤 (충남대학교 수의과대학) ;
  • 조성환 (충남대학교 수의과대학) ;
  • 김무강 (충남대학교 수의과대학) ;
  • 김성호 (전남대학교 수의과대학) ;
  • 권오덕 (전북대학교 수의과대학)
  • 발행 : 1997.03.01

초록

흰쥐 간정맥 및 간문맥에 PE10 튜브를 마취하에 이식시킨 후 5일째부터 고형사료와 10% creatine을 함유한 고형사료를 5일 동안 섭식 시켜 급속 냉동 시켰다. 냉동시킨 흰쥐 간장, 뇌 및 근육에서 creatine을 포함한 간장 metabolites을 조사하여 다음과 같은 결과를 얻었다. 또한 간장세포내의 water compartment의 변화를 동시에 실시 하였다. Creatine을 섭식시킨 실험군은 정상 대조군과 비교하여 섭식에 의한 간세포내의 water compartment 에 벼노하를 주지 않았고 간장 세포내의 creatine농도에 있어서는 약 43배 이상 세포내 증가를 유도하였으며, 이는 세포외 및 세포내의 creatine의 gradient에 의해 영향을 받고 있음을 알 수가 있었고, 또한 동시에 뇌, 근육 등에서는 섭식에 의해 유의성 있는 농도 변화는 보이지 않았다. 이는 세포 외액속에 함유된 creatine 이 여러 수송기전에 의하여 간세포내로 흡착이 이루어진 것을 알 수 있었으나, 간세포 분리 시험법에로 Creatine Kinase 효소 활성도를 측정한 결과 non-parenchymal hepatocytes에서만 거의 100% 효소 활성도가 있음을 증명 하였다.

키워드

참고문헌

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